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| Status |
Public on Apr 14, 2015 |
| Title |
Molecular Signature of Erythroblast Enucleation in Human Embryonic Stem Cells |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organisms |
Homo sapiens; synthetic construct |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
While enucleation is a critical step in the terminal differentiation of human red blood cells, the molecular mechanisms underlying this unique process remain unclear. To investigate erythroblast enucleation, we studied the erythroid differentiation of human embryonic stem cells (hESCs), which provide a unique model for deeper understanding of the development and differentiation of multiple cell types. First, using a two-step protocol, we demonstrated that terminal erythroid differentiation from hESCs is directly dependent on the age of the embryoid bodies. Second, by choosing hESCs in two extreme conditions of erythroid culture, we obtained
an original differentiation model which allows one to study the mechanisms underlying the enucleation of erythroid cells by analyzing the gene and miRNA (miR) expression profiles of cells from these two culture conditions. Third, using an integrated analysis of mRNA and miR expression profiles, we identified five miRs potentially involved in erythroblast enucleation.
Finally, by selective knockdown of these five miRs we found miR-30a to be a regulator of erythroblast enucleation in hESCs.
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| Overall design |
Refer to individual Series
|
| |
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| Citation(s) |
25850942 |
| |
| Submission date |
Mar 03, 2014 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Hélène LAPILLONNE |
| E-mail(s) |
helene.lapillonne@trs.aphp.fr
|
| Organization name |
UPMC Univ Paris 06
|
| Department |
UMR_S938 CDR Saint-Antoine
|
| Lab |
Prolifération et Différentiation des Cellules Souches
|
| Street address |
27 Rue de Chaligny
|
| City |
Paris |
| ZIP/Postal code |
F-75012 |
| Country |
France |
| |
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| Platforms (2) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
| GPL15235 |
miRXplore TM Microarray (958) |
|
| Samples (24)
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| This SuperSeries is composed of the following SubSeries: |
| GSE55530 |
Molecular Signature of Erythroblast Enucleation in Human Embryonic Stem Cells |
| GSE55531 |
Molecular Signature of Erythroblast Enucleation in Human Embryonic Stem Cells |
|
| Relations |
| BioProject |
PRJNA239880 |
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