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| Status |
Public on Jul 06, 2014 |
| Title |
Role of Polycomb group proteins in the DNA damage response – a reassessment |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We have investigated whether components of Polycomb repressive complex 1 (PRC1) are recruited to double-strand breaks (DSBs) generated by inducible expression of the AsiSI restriction enzyme in normal human fibroblasts. Using chromatin immunoprecipitation and deep sequencing (ChIP-seq), which detects PRC1 proteins at common sites throughout the genome, we did not find evidence for recruitment of PRC1 components to AsiSI-induced DSBs. In contrast, the S2056 phosphorylated form of DNA-PKcs and other DNA repair proteins were detected at a subset of AsiSI sites that are predominantly at the 5’ ends of transcriptionally active genes. Our data question the idea that PcG protein recruitment provides a link between DSB repairs and transcriptional repression.
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| Overall design |
Single chromatin preparations from treated and untreated cells (+/- OHT), immunoprecipitated with two antibodies (pDNA-PKcs and MEL18) were sequenced as technical replicates, along with the corresponding input DNAs
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| Contributor(s) |
Chandler H, Patel H, Palermo R, Brookes S, Matthews N, Peters G |
| Citation(s) |
25057768 |
| |
| Submission date |
Mar 05, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gordon Peters |
| E-mail(s) |
gordon.peters@cancer.org.uk
|
| Phone |
+44 207 269 3049
|
| Organization name |
CRUK London Research Institute
|
| Lab |
Molecular Oncology
|
| Street address |
44 Lincolns Inn Fields
|
| City |
London |
| ZIP/Postal code |
WC2A 3LY |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| GSM1340089 |
DNAPKCS METHANOL HS ChipSeq technical replicate 1 and 2 |
| GSM1340090 |
DNAPKCS OHT HS ChipSeq technical replicate 1 and 2 |
| GSM1340091 |
MEL18 METHANOL HS ChipSeq technical replicate 1 and 2 |
| GSM1340092 |
MEL18 OHT HS ChipSeq technical replicate 1 and 2 |
| GSM1340093 |
INPUT METHANOL HS ChipSeq technical replicate 1 and 2 |
| GSM1340094 |
INPUT OHT HS ChipSeq technical replicate 1 and 2 |
|
| Relations |
| BioProject |
PRJNA240224 |
| SRA |
SRP039445 |
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