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Series GSE55812 Query DataSets for GSE55812
Status Public on Mar 12, 2014
Title Gene expression in IL-10-deficient and wild-type mice following Citrobacter rodentium infection
Organism Mus musculus
Experiment type Expression profiling by array
Summary To further understand immune mechanims involved in regulating intestinal inflammation, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential of regulating inflammation in the absence of IL-10. Whole colon tissue from IL-10-deficient and C57BL/6 (wild-type) mice was collected 2 weeks after Citrobacter rodentium infection and from uninfected controls. Consistent with the histological and cellular analysis, expression levels of many chemokines and cytokines involved in recruiting leukocytes and promoting inflammation were, on average, lower in IL-10 deficient compared to wild-type mice after infection. An exception to this general trend was IL-27, a cytokine with both pro- and anti-inflammatory properties.
 
Overall design Two weeks after Citrobacter rodentium challenge, total RNA was extracted and analyzed from whole colon tissue of infected IL-10-deficient and wild-type mice, and compared to uninfected controls. Each sample contained equal amounts of total RNA from 4-5 female mice which were pooled and used in the experiment.
 
Contributor(s) Dann SM, Eckmann L
Citation(s) 24566625
Submission date Mar 11, 2014
Last update date Jan 12, 2017
Contact name Lars Eckmann
E-mail(s) leckmann@ucsd.edu
Organization name University of San Diego
Department Gastroenterology
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093-0063
Country USA
 
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (4)
GSM1346306 C57BL/6_Uninfected
GSM1346307 C57BL/6_Infected
GSM1346308 IL-10-deficient_Uninfected
Relations
BioProject PRJNA241040

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55812_RAW.tar 32.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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