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Status |
Public on Apr 08, 2014 |
Title |
PAX6 Regulates Melanogenesis in the Retinal Pigmented Epithelium through Feed-Forward Regulatory Interactions with MITF |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
During organogenesis, PAX6 is required for establishment of various progenitor subtypes within the central nervous system, eye and pancreas. PAX6 expression is maintained in a variety of cell types within each organ, although its role in each lineage and how it acquires cell-specific activity remain elusive. Herein, we aimed to determine the roles and the hierarchical organization of the PAX6-dependent gene regulatory network during the differentiation of the retinal pigmented epithelium (RPE). Somatic mutagenesis of Pax6 in the differentiating RPE revealed that PAX6 functions in a feed-forward regulatory loop with MITF during onset of melanogenesis. PAX6 both controls the expression of an RPE isoform of Mitf and synergizes with MITF to activate expression of genes involved in pigment biogenesis. This study exemplifies how one kernel gene pivotal in organ formation accomplishes a lineage-specific role during terminal differentiation of a single lineage.
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Overall design |
The data contains 3 control and 3 Pax6 negative samples representing biological repeats. Each sample was prepared from RPE dissected from E15.5 transgenic animals, either Pax6loxP/loxP or Pax6loxP/loxP;DctCre.
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Contributor(s) |
Raviv S, Padan RA |
Citation(s) |
24875170 |
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Submission date |
Apr 07, 2014 |
Last update date |
Mar 04, 2019 |
Contact name |
Ruth Ashery-Padan |
E-mail(s) |
ruthash@post.tau.ac.il
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Organization name |
Tel-Aviv University
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Department |
Department of Human Molecular Genetics and Biochemistry
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Lab |
Ruth Ashery-Padan
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Street address |
Tel Aviv University
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City |
Tel Aviv |
State/province |
Select a State or Province |
ZIP/Postal code |
69978 |
Country |
Israel |
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Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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Samples (6)
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Relations |
BioProject |
PRJNA243880 |