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Series GSE56548 Query DataSets for GSE56548
Status Public on Apr 08, 2014
Title PAX6 Regulates Melanogenesis in the Retinal Pigmented Epithelium through Feed-Forward Regulatory Interactions with MITF
Organism Mus musculus
Experiment type Expression profiling by array
Summary During organogenesis, PAX6 is required for establishment of various progenitor subtypes within the central nervous system, eye and pancreas. PAX6 expression is maintained in a variety of cell types within each organ, although its role in each lineage and how it acquires cell-specific activity remain elusive. Herein, we aimed to determine the roles and the hierarchical organization of the PAX6-dependent gene regulatory network during the differentiation of the retinal pigmented epithelium (RPE). Somatic mutagenesis of Pax6 in the differentiating RPE revealed that PAX6 functions in a feed-forward regulatory loop with MITF during onset of melanogenesis. PAX6 both controls the expression of an RPE isoform of Mitf and synergizes with MITF to activate expression of genes involved in pigment biogenesis. This study exemplifies how one kernel gene pivotal in organ formation accomplishes a lineage-specific role during terminal differentiation of a single lineage.
 
Overall design The data contains 3 control and 3 Pax6 negative samples representing biological repeats. Each sample was prepared from RPE dissected from E15.5 transgenic animals, either Pax6loxP/loxP or Pax6loxP/loxP;DctCre.
 
Contributor(s) Raviv S, Padan RA
Citation(s) 24875170
Submission date Apr 07, 2014
Last update date Mar 04, 2019
Contact name Ruth Ashery-Padan
E-mail(s) ruthash@post.tau.ac.il
Organization name Tel-Aviv University
Department Department of Human Molecular Genetics and Biochemistry
Lab Ruth Ashery-Padan
Street address Tel Aviv University
City Tel Aviv
State/province Select a State or Province
ZIP/Postal code 69978
Country Israel
 
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM1363570 E15.5 RPE_control_Rep1
GSM1363571 E15.5 RPE_control_Rep2
GSM1363572 E15.5 RPE_control_Rep3
Relations
BioProject PRJNA243880

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56548_KOvsCON_processed_data_p_0_05_FC_1_5.txt.gz 7.0 Kb (ftp)(http) TXT
GSE56548_RAW.tar 22.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Processed data are available on Series record

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