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Series GSE56889 Query DataSets for GSE56889
Status Public on Nov 07, 2014
Title Genome-wide location of histone acetyltransferase Sas3 from Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by array
Summary We have investigated the genome-wide occupancy of Sas3p by ChIP-Chip, using tiled microarrays. Using this technique, it has been described that H3K14 and H3K9 acetylation is enriched at promoter regions and transcriptional start sites of active genes. Considering that Sas3p is a HAT whose main target in vitro is H3K14 we expected to detect Sas3 binding largely to promoter regions of genes. Surprisingly, we found that Sas3p is associated to the coding regions of genes, with a peak enrichment located) within the 5’ half of the ORF, and this enrichment drops substantially toward the 3’ region of the ORF. This result is very similar to that obtained for Yng1 genome-wide occupancy, also a component of the NuA3 complex, suggesting that this complex could be involved in transcriptional elongation, at least, in an initial step of the process.
Overall design The experiments described in this study compare ChIP with an anti-HA antibody of a SAS3-HA tagged strain (BQS1217) to a control ChIP with an anti-HA antibody of a wild type strain (BY4742). Budding yeast samples were analyzed in exponential growing conditions (YPD). 2 biological replicates were performed.
Contributor(s) Pamblanco M, Tordera V
Citation(s) 25473596
Submission date Apr 17, 2014
Last update date Feb 05, 2015
Contact name Vicent Pelechano
Organization name ScilifeLab - Karolinska Institutet
Department MTC
Street address Nobels väg 16
City Solna
ZIP/Postal code SE-17177
Country Sweden
Platforms (1)
GPL4130 Agilent-014741 Yeast Whole Genome ChIP-on-Chip Microarray 244K (G4491A)
Samples (2)
GSM1370711 SAS3-HA_YPD_rep1
GSM1370712 SAS3-HA_YPD_rep2
BioProject PRJNA244868

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Supplementary file Size Download File type/resource
GSE56889_RAW.tar 131.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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