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Series GSE57062 Query DataSets for GSE57062
Status Public on May 01, 2014
Title Improved tagmentation-based whole genome bisulfite sequencing for input DNA from less than 100 cells
Organism Oryza sativa
Experiment type Methylation profiling by high throughput sequencing
Summary We improved the tagmentation-based whole genome bisulfite sequencing technology, enabling stable library construction with complexity from minimally 0.5ng of initial genomic DNA, which equals less than 100 cells. This new approach is highly attractive for the complete methylome analysis of very limited cell numbers, e.g., pre-implantation embryos, or tiny biopsy specimens.
 
Overall design Applying this newly modified T-WGBS, we sequenced the methylome of a rice strain using libraries constructed from 10 ng, 1 ng and 0.5 ng of input genomic DNA. Two libraries were independently constructed and sequenced for each aliquot of input genomic DNA
 
Contributor(s) Gao F, Lu H
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Apr 24, 2014
Last update date May 15, 2019
Contact name Desheng Gong
E-mail(s) gds19870718@163.com
Organization name Agricultural Genomes Institute at Shenzhen
Street address No.7 PengFei road
City Shenzhen
ZIP/Postal code 518120
Country China
 
Platforms (1)
GPL13160 Illumina HiSeq 2000 (Oryza sativa)
Samples (7)
GSM1373863 LCBS-seq
GSM1373864 TBS-seq for 10ng repeat1
GSM1373865 TBS-seq for 10ng repeat2
Relations
BioProject PRJNA245358
SRA SRP041428

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57062_RAW.tar 5.2 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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