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Series GSE57228 Query DataSets for GSE57228
Status Public on Apr 01, 2015
Title ChIP-seq of E4F1 in primary mouse embryonic fibroblasts (MEFs) and in p53-/-, Ha-RasV12-transformed MEFs
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary E4F1 is a ubiquitously expressed zinc-finger protein of the Gli-Kruppel family that was first identified, more than 30 years ago, as a cellular target of the adenoviral oncoprotein E1A13S (Ad type V), required for transcriptional regulation of adenoviral genes. In order to decipher E4F1 cellular target genes, we performed chromatin immunoprecipitation of endogenous E4F1 in primary and in p53KO, Ha-RasV12-transformed MEFs. Both input and immunoprecipitated DNA were exhaustively sequenced and mapped on the mouse genome (mm9). Peak detection has been achieved by combining two peak calling algorithms. Intersection of the two E4F1 peak lists on each cell line were considered as E4F1 chromatin bound regions.
 
Overall design Genome-wide mapping of E4F1 binding in mouse embryonic fibroblasts.
 
Contributor(s) Kirsh O, Rodier G, Sardet C
Citation(s) 25843721, 26484288, 27621446, 27621431
Submission date May 01, 2014
Last update date May 15, 2019
Contact name Claude Sardet
E-mail(s) claude.sardet@inserm.fr
Organization name Institut de Recherche en Cancérologie de Montpellier (IRCM)
Department Inserm U1194 - Université de Montpellier - Institut Régional du Cancer de Montpellier (ICM)
Street address 208 rue des Apothicaires
City Montpellier
ZIP/Postal code 34298
Country France
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (4)
GSM1377538 ChIP E4F1 in primary MEF
GSM1377539 Primary MEF Input
GSM1377540 ChIP E4F1 in p53KORasV12 MEF
This SubSeries is part of SuperSeries:
GSE57242 The transcription factor E4F1 coordinates CHK1-dependent checkpoint and mitochondrial functions
Relations
BioProject PRJNA246035
SRA SRP041609

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57228_RAW.tar 275.6 Mb (http)(custom) TAR (of BAR, BED, COD, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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