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Series GSE57241 Query DataSets for GSE57241
Status Public on Dec 17, 2014
Title RNF17 referees ping-pong in mouse testes [RIP-seq]
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary In mammals, piRNA populations are dynamic throughout male germ cell development. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, however, the piRNA population is transposon-poor and restricted to primary piRNAs derived from pachytene piRNA clusters1-6. The mechanism controlling which piRNAs are present at each developmental stage is poorly understood. Here we show that RNF17 shapes adult meiotic piRNA content by suppressing the production of secondary piRNAs. In the absence of RNF17, ping-pong (secondary amplification) occurs inappropriately in meiotic cells – aberrantly targeting protein-coding genes and lncRNAs. Our data indicate that RNF17 comprises one component of a “refereeing” mechanism that prevents deleterious activity of the meiotic piRNA pathway by ensuring the selective loading of PIWI proteins with products of meiotic piRNA clusters.
Overall design Examination of small RNAs isolated from MIWI and MILI IPs of heterozygous and homozygous RNF17 adult testes
Contributor(s) Wasik KA, Tam O, Hao Y, Falciatori I, Hammell M, Vagin VV, Hannon GJ
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Submission date May 01, 2014
Last update date May 15, 2019
Contact name Vasily Vagin
Organization name Cold Spring Harbor
Lab Hannon's
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (4)
GSM1377730 MILI_IP_het_RNF17 testes
GSM1377731 MIWI_IP_het_RNF17 testes
GSM1377732 MILI_IP_homtRNF17 testes
This SubSeries is part of SuperSeries:
GSE53919 RNF17 blocks promiscuous activity of PIWI proteins in mouse testes
BioProject PRJNA246031
SRA SRP041607

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57241_RAW.tar 1.2 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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