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Series GSE57395 Query DataSets for GSE57395
Status Public on Jul 14, 2014
Title Direct Induction of Hematoendothelial Program in Human Pluripotent Stem Cells by Transcriptional Regulators
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Advancing pluripotent stem cell technologies for modeling hematopoietic stem cell development and therapies requires identifying key regulators of hematopoietic commitment from human pluripotent stem cells (hPSCs). Here, by screening the effect of 27 candidate factors, we identified two groups of transcriptional regulators capable of inducing distinct hematopoietic programs from hPSCs: pan-myeloid (GATA2 and ETV2) and erythro-megakaryocytic (GATA2 and TAL1). In both cases, these transcription factors directly converted hPSCs to endothelium, which subsequently transformed into blood cells with pan-myeloid or eryhtro-megakaryocytic potential. These data demonstrate that two distinct genetic programs regulate the hematopoietic development from hPSCs and that both of these programs specify hPSCs directly to hemogenic endothelial cells. Additionally, this study provides a novel method for efficient induction of blood and endothelial cells from hPSCs via overexpression of modified mRNA for selected transcription factors.
Overall design mRNA profiles of 54 samples, including 4 samples in duplicate and 6 control samples were generated by deep sequencing, using Illumina HiSeq 2500.
Contributor(s) Elcheva I, Volchanskaya VB, Swanson S, Stewart R, Thomson J, Slukvin I
Citation(s) 25019369
Submission date May 07, 2014
Last update date May 15, 2019
Contact name Scott Swanson
Organization name Morgridge Institute for Research
Lab Thomson
Street address 330 N. Orchard St
City Madison
State/province WI
ZIP/Postal code 53715
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (54)
GSM1381984 hESC_GFP
GSM1381985 hESC_1
GSM1381986 hESC_2
BioProject PRJNA246387
SRA SRP041751

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