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| Status |
Public on Oct 01, 2006 |
| Title |
Gene Expression Profiling of Familial and Sporadic Cases of Interstitial Pneumonia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Rationale: Idiopathic interstitial pneumonia (IIP) and its’ familial variants are progressive and largely untreatable disorders with poorly understood molecular mechanisms. Both the genetics and the histologic type of IIP play a role in understanding the etiology and pathogenesis of interstitial lung disease, but transcriptional signatures of these subtypes are unknown.
Objectives: To evaluate gene expression in the lung tissue from patients with usual interstitial pneumonia (UIP) and non-specific interstitial pneumonia (NSIP) that were either familial or non-familial in origin and compare them to gene expression from normal lung parenchyma.
Methods: We profiled RNA from lungs of 16 patients with sporadic IIP, 10 with familial IIP, and 9 normal controls on a whole human genome oligonucleotide microarray.
Results: Significant transcriptional differences exist in familial and sporadic IIPs. The genes distinguishing the genetic subtypes belong to the same functional categories as transcripts that distinguish IIP from normal samples. Relevant categories include chemokines and growth factors and their receptors, complement components, genes associated with cell proliferation and death, and genes in the Wnt pathway.
Keywords: disease state analysis
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| Overall design |
35 lung RNA samples were profiled against universal human RNA reference (Stratagene) in duplicate with Cy3 and Cy5 dyes swapped. 9 samples are normal lung tissue and 26 samples are subjects with idiopathic interstitial pneumonia (10 have familal form of the disease and 16 have sporadic IIP).
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| Contributor(s) |
Yang IV, Burch LH, Steele MP, Savov JD, Hollingsworth JW, McElvania-Tekippe E, Berman KG, Speer MC, Sporn TA, Brown KK, Schwarz MI, Schwartz DA |
| Citation(s) |
16998095 |
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| Submission date |
Sep 06, 2006 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Ivana V Yang |
| E-mail(s) |
yangi@njhealth.org
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| Phone |
303-270-2589
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| Fax |
303-270-2136
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| Organization name |
National Jewish Health
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| Department |
Medicine
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| Street address |
1400 Jackson Street
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| City |
Denver |
| State/province |
CO |
| ZIP/Postal code |
80206 |
| Country |
USA |
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| Platforms (1) |
| GPL4255 |
NIH-NIEHS/Agilent Human Familial IIP 43K array |
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| Samples (70)
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| Relations |
| BioProject |
PRJNA97121 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5774_RAW.tar |
696.4 Mb |
(http)(custom) |
TAR (of TXT) |
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