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Series GSE58034 Query DataSets for GSE58034
Status Public on Jun 06, 2014
Title The SET-domain protein SUVR5 mediates H3K9me2 deposition and silencing at stimulus response genes in a DNA methylation independent manner [SELEX]
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary In eukaryotic cells, environmental and developmental signals alter chromatin structure and modulate gene expression. Heterochromatin constitutes the transcriptionally inactive state of the genome and in plants and mammals is generally characterized by DNA methylation and histone modifications such as histone H3 lysine 9 (H3K9) methylation. In Arabidopsis thaliana DNA methylation and H3K9 methylation are usually colocated and set up a mutually self reinforcing and stable state. Here, in contrast, we found that SUVR5, a plant Su(var)3-9 homolog with a SET histone methyltransferase domain, mediates H3K9me2 deposition and regulates gene expression in a DNA-methylation-independent manner. SUVR5 binds DNA through its zinc fingers and represses the expression of a subset of stimulus response genes. This represents a novel mechanism for plants to regulate their chromatin and transcriptional state, which may allow for the adaptability and modulation necessary to rapidly respond to extracellular cues.
 
Overall design one experiment and one control.
 
Contributor(s) Caro E, Jacobsen S, Stroud H
Citation(s) 23071452
Submission date May 28, 2014
Last update date May 15, 2019
Contact name Hume Stroud
Organization name UT Southwestern
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
 
Platforms (1)
GPL9062 Illumina Genome Analyzer (Arabidopsis thaliana)
Samples (2)
GSM1399652 gSELEXx1
GSM1399653 gSELEXx9
This SubSeries is part of SuperSeries:
GSE39405 The SET-domain protein SUVR5 mediates H3K9me2 deposition and silencing at stimulus response genes in a DNA methylation independent manner
Relations
BioProject PRJNA251822
SRA SRP042996

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE58034_gSELEXx9_vs_gSELEXx1_peaks.bed.gz 15.4 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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