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Status |
Public on Jun 12, 2014 |
Title |
Target repression induced by endogenous microRNAs: large differences, small effects |
Organism |
Drosophila melanogaster |
Experiment type |
Non-coding RNA profiling by array
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Summary |
MicroRNAs are small RNAs that regulate protein levels. It is commonly assumed that the expression level of a microRNA is directly correlated with its repressive activity – that is, highly expressed microRNAs will repress their target mRNAs more. Here we investigate the quantitative relationship between endogenous microRNA expression and repression for 32 mature microRNAs in Drosophila melanogaster S2 cells. In general, we find that more abundant microRNAs repress their targets to a greater degree. However, the relationship between expression and repression is nonlinear, such that a 10-fold greater microRNA concentration produces only a 10% increase in target repression. The expression/repression relationship is the same for both dominant guide microRNAs and minor mature products (so-called passenger strands/microRNA* sequences). However, we find examples of microRNAs whose cellular concentrations differ by several orders of magnitude, yet induce similar repression of target mRNAs. Likewise, microRNAs with similar expression can have very different repressive abilities. We show that the association of microRNAs with Argonaute proteins does not explain this variation in repression. The observed relationship is consistent with the limiting step in target repression being the association of the microRNA/RISC complex with the target site. These findings argue that modest changes in cellular microRNA concentration will have minor effects on repression of targets.
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Overall design |
Two replicates of S2-DRSC cells under normal conditions
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Contributor(s) |
Hunt SM |
Citation missing |
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Submission date |
Jun 11, 2014 |
Last update date |
Jun 13, 2014 |
Contact name |
Suzanne Hunt |
Organization name |
University of Manchester
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Department |
Life Sciences
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Lab |
Matthew Ronshaugen
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Street address |
Dover Street
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City |
Manchester |
ZIP/Postal code |
M13 9PT |
Country |
United Kingdom |
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Platforms (1) |
GPL18786 |
LC Sciences MRA-1006_miRDrosophilaM_17 |
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Samples (2) |
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Relations |
BioProject |
PRJNA252528 |
Supplementary file |
Size |
Download |
File type/resource |
GSE58415_Normalized_Data.xls.gz |
87.3 Kb |
(ftp)(http) |
XLS |
GSE58415_RAW.tar |
150.0 Kb |
(http)(custom) |
TAR (of TXT) |
GSE58415_statistical_analysis_T-test-01-S2-2_vs_S2-1.txt.gz |
11.1 Kb |
(ftp)(http) |
TXT |
Processed data included within Sample table |
Processed data are available on Series record |
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