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Series GSE58415 Query DataSets for GSE58415
Status Public on Jun 12, 2014
Title Target repression induced by endogenous microRNAs: large differences, small effects
Organism Drosophila melanogaster
Experiment type Non-coding RNA profiling by array
Summary MicroRNAs are small RNAs that regulate protein levels. It is commonly assumed that the expression level of a microRNA is directly correlated with its repressive activity – that is, highly expressed microRNAs will repress their target mRNAs more. Here we investigate the quantitative relationship between endogenous microRNA expression and repression for 32 mature microRNAs in Drosophila melanogaster S2 cells. In general, we find that more abundant microRNAs repress their targets to a greater degree. However, the relationship between expression and repression is nonlinear, such that a 10-fold greater microRNA concentration produces only a 10% increase in target repression. The expression/repression relationship is the same for both dominant guide microRNAs and minor mature products (so-called passenger strands/microRNA* sequences). However, we find examples of microRNAs whose cellular concentrations differ by several orders of magnitude, yet induce similar repression of target mRNAs. Likewise, microRNAs with similar expression can have very different repressive abilities. We show that the association of microRNAs with Argonaute proteins does not explain this variation in repression. The observed relationship is consistent with the limiting step in target repression being the association of the microRNA/RISC complex with the target site. These findings argue that modest changes in cellular microRNA concentration will have minor effects on repression of targets.
 
Overall design Two replicates of S2-DRSC cells under normal conditions
 
Contributor(s) Hunt SM
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Submission date Jun 11, 2014
Last update date Jun 13, 2014
Contact name Suzanne Hunt
Organization name University of Manchester
Department Life Sciences
Lab Matthew Ronshaugen
Street address Dover Street
City Manchester
ZIP/Postal code M13 9PT
Country United Kingdom
 
Platforms (1)
GPL18786 LC Sciences MRA-1006_miRDrosophilaM_17
Samples (2)
GSM1410563 S2-DRSC 1
GSM1410564 S2-DRSC 2
Relations
BioProject PRJNA252528

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58415_Normalized_Data.xls.gz 87.3 Kb (ftp)(http) XLS
GSE58415_RAW.tar 150.0 Kb (http)(custom) TAR (of TXT)
GSE58415_statistical_analysis_T-test-01-S2-2_vs_S2-1.txt.gz 11.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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