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Series GSE58614 Query DataSets for GSE58614
Status Public on Jun 19, 2014
Title Expression profiling of Aldh1l1-precursors in the developing spinal cord reveals glial lineage-specific genes and direct Sox9-Nfe2l1 interactions
Organism Mus musculus
Experiment type Expression profiling by array
Summary Developmental regulation of gliogenesis in the mammalian CNS is incompletely understood, in part due to a limited repertoire of lineage-specific genes. We used Aldh1l1-GFP as a marker for gliogenic radial glia and later-stage precursors of developing astrocytes and performed gene expression profiling of these cells. We then used this dataset to identify candidate transcription factors that may serve as glial markers or regulators of glial fate. Our analysis generated a database of developmental stage-related markers of Aldh1l1+ cells between murine embryonic day 13.5-18.5. Using these data we identify the bZIP transcription factor Nfe2l1 and demonstrate that it promotes glial fate under direct Sox9 regulatory control. Thus, this dataset represents a resource for identifying novel regulators of glial development.
Overall design 18 total samples consisting of three biological replicates each of flow sorted embryonic spinal cord Aldh1l1-GFP positive cells and whole cord, spanning the radial glial to astrocyte transition
Contributor(s) Molofsky AV, Oldham MC, Rowitch DH
Citation(s) 23840004
Submission date Jun 18, 2014
Last update date Mar 04, 2019
Contact name David Rowitch
Organization name University of California-San Francisco
Department Institute for Regenerative Medicine
Lab Rowitch
Street address 35 Medical Center Way, RMB 932D
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (18)
GSM1415459 Aldh1l1-GFP positive cells, age E13.5 biological rep. 1
GSM1415460 Aldh1l1-GFP positive cells, age E13.5 biological rep. 2
GSM1415461 Aldh1l1-GFP positive cells, age E13.5 biological rep. 3
BioProject PRJNA253023

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MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE58614_RAW.tar 78.2 Mb (http)(custom) TAR (of CEL)
GSE58614_coexpression_ME_network_19-35-20.pdf.gz 3.3 Kb (ftp)(http) PDF
GSE58614_coexpression_Module_statistics_19-35-20.xlsx.gz 35.5 Kb (ftp)(http) XLSX
GSE58614_coexpression_Modules_19-35-20.pdf.gz 290.3 Kb (ftp)(http) PDF
GSE58614_coexpression_kME_table_19-35-20.xlsx.gz 8.0 Mb (ftp)(http) XLSX
GSE58614_differential_expression_AvsW_13.5.xlsx.gz 647.9 Kb (ftp)(http) XLSX
GSE58614_differential_expression_AvsW_14.5.xlsx.gz 215.9 Kb (ftp)(http) XLSX
GSE58614_differential_expression_AvsW_17.5.txt.gz 19.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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