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Series GSE59309 Query DataSets for GSE59309
Status Public on Jul 10, 2014
Title Multicilin drives centriole biogenesis via E2f proteins
Organism Xenopus laevis
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Biochemistry suggests e2f4 forms a complex with the coiled-coiled protein multicilin (MCIDAS), a protein that is necessary and sufficient to specify multiciliated cells in vertebrates. Here, we performed RNAseq on Xenopus laevis ectoderm in the presence of multicilin alone or multicilin and a dominant-negative e2f4 construct. We also performed ChIPseq on e2f4 in the presence or absence of multicilin. Taken together, these data demonstrate how multicilin affects e2f4 genomic targets and their downstream transcription.
Overall design RNAseq: misexpression of multicilin-HGR +/- dominant-negative e2f4 messenger RNAs in X. laevis animal caps, multicilin induced with dexamethasone at mid-stage 11 and harvested at 3 timepoints (3, 6, and 9 hours after induction, roughly corresponding to stages 13, 16, and 18) with 3 biological replicates.
ChIPseq: misexpression of e2f4-GFP +/- multicilin-HGR messenger RNAs in X. laevis animal caps, multicilin induced at mid-stage 11 and harvested at one timepoint (6 hours after induction, roughly corresponding to stage 16), immunoprecipitated with anti-GFP and sequenced; 2 biological replicates. Background was input prior to IP.
Contributor(s) Ma L, Quigley IK, Kintner C
Citation(s) 24934224
Submission date Jul 10, 2014
Last update date May 15, 2019
Contact name Ian K Quigley
Phone 858-453-4100
Organization name Salk Institute
Department Molecular Neurobiology Lab
Lab Kintner
Street address 10010 North Torrey Pines Road
City La Jolla
State/province CA
ZIP/Postal code 92037
Country USA
Platforms (1)
GPL18936 Illumina HiSeq 2500 (Xenopus laevis)
Samples (22)
GSM1434771 MCIDAS_3h_RNAseq_rep_1
GSM1434772 MCIDAS_3h_RNAseq_rep_2
GSM1434773 MCIDAS_3h_RNAseq_rep_3
BioProject PRJNA255038
SRA SRP044238

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Supplementary file Size Download File type/resource
GSE59309_RAW.tar 34.0 Mb (http)(custom) TAR (of BED, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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