|
| Status |
Public on Jan 01, 2007 |
| Title |
High-salt diet leads to differential gene expression in DOCA-treated mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In the present study we made use of the (1-renin) DOCA-salt mouse model - which has been previously shown to develop cardiac and renal hypertrophy - to evaluate the direct effects of high-salt diet on cardiac function and gene expression profiling. The comparison between low-salt and high-salt DOCA-treated mice will reveal what genes are directly modulated by sodium in (normotensive) DOCA-treated mice.
Previous publications:
Wang Q, Hummler E, Nussberger J, Clement S, Gabbiani G, Brunner HR,
Burnier M. Blood pressure, cardiac, and renal responses to salt and
deoxycorticosterone acetate in mice: role of renin genes. J Am Soc
Nephrol. 2002;13:1509 –1516.
Wang Q, Domenighetti AA, Pedrazzini T, Burnier M. Potassium supplementation reduces cardiac and renal hypertrophy independent of blood pressure in DOCA/salt mice. Hypertension. 2005 Sep;46(3):547-54.
Keywords: comparative dose-response treatment (2 groups)
|
| |
|
| Overall design |
In this first series, six (7-8 week old) DOCA-treated mice were separated in 2 groups:
Group1 (Low-Salt)= LS + Nx + DOCA35 + tap
(LS= 0.5mg Na/g food; Nx= uninephrectomized; DOCA35= deoxycorticosterone acetate for 5 weeks; tap= normal drinking water)
Group2 (High-salt)= LS + Nx + DOCA35 + 1%NaCl
(LS= 0.5mg Na/g food; Nx= uninephrectomized; DOCA35= deoxycorticosterone acetate for 5 weeks; 1%NaCl= 1%NaCl in drinking water for 5 weeks)
cDNA high-density microarray assays were used to explore changes in cardiac expression of ~17’000 clones in hearts of Low-salt and High-salt treated DOCA-mice.
This series contains 3 biological replicates and one dye swap.
|
| |
|
| Contributor(s) |
Domenighetti AA, Wang Q, Burnier M |
| Citation(s) |
22403797 |
| |
| Submission date |
Oct 02, 2006 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Andrea A. Domenighetti |
| E-mail(s) |
domenigo@yahoo.com
|
| Organization name |
Northwestern University & Rehabilitation Institute of Chicago
|
| Lab |
Room 1396
|
| Street address |
345 East Superior St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (4)
|
| GSM138330 |
Heart_DOCA_High_vs._Low_Salt_M17ka_859 |
| GSM138331 |
Heart_DOCA_High_vs._Low_Salt_M17ka_860 |
| GSM138332 |
Heart_DOCA_High_vs._Low_Salt_M17ka_861 |
| GSM138333 |
Heart_DOCA_High_vs._Low_Salt_M17ka_862 |
|
| Relations |
| BioProject |
PRJNA97399 |
Less...
More...