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Series GSE59533 Query DataSets for GSE59533
Status Public on Feb 23, 2015
Title Expression data from Zea mays cultivars Tietar and DKC 6575
Organism Zea mays
Experiment type Expression profiling by array
Summary Maize transgenic event MON810, grown and commercialised worldwide, is the only cultivated GM event in EU. Maize MON810, variety DKC6575, and the corresponding near-isogenic Tietar were studied in different growing conditions, to assess their behaviour in response to drought. Profiling gene expression in water deficit regimes and in generalised water stress showed an up-regulation of different stress- responsive genes. A greater number of differentially expressed genes was observed in Tietar rather than in DKC6575, with genes belonging to transcription factor families and genes encoding HSPs, LEAs and detoxification enzymes. Since these genes have been from literature, indicated as typical of stress responses, their activation in Tietar rather than in DKC6575 may be reminiscent of a more efficient water stress response. DKC6575 was also analysed for the expression of the transgene CryIAb (encoding for the delta-endotoxin insecticidal protein) in water limiting conditions. In all the experiments the CryIAb transcript was not influenced by water stress, but expressed at a constant level. This suggests that though a different pattern of sensitivity to stress, the transgenic variety maintains the same expression level for the transgene.
 
Overall design The Maize Oligonucleotide microarray was used to measure differences in gene expression levels under drought stress in DKC6575 and in the near-isogenic variety Tietar growing in the field. The gene expression profile of each variety was evaluated at T1 and at T2 for samples fully irrigated and at T2 for samples with irrigation deficit.
 
Contributor(s) Gullì M, Marmiroli N
Citation(s) 25692547
Submission date Jul 17, 2014
Last update date Feb 24, 2015
Contact name Mariolina Gulli
Organization name University of Parma
Department Life Sciences
Street address Parco Area delle Scienze 11a
City Parma
ZIP/Postal code 43124
Country Italy
 
Platforms (1)
GPL4032 [Maize] Affymetrix Maize Genome Array
Samples (6)
GSM1438946 DKC6575_leaves at T1 fully irrigated
GSM1438947 Tietar_leaves at T1 fully irrigated
GSM1438948 DKC6575_leaves at T2 fully irrigated
Relations
BioProject PRJNA255511

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59533_RAW.tar 11.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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