|
| Status |
Public on Apr 01, 2015 |
| Title |
Striatal genes regulated by super-enhancers and displaying low paused RNAPII are preferentially down-regulated in Huntington’s disease [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Huntington neurodegenerative disease (HD) is associated with extensive down-regulation of neuronal genes. We show preferential down-regulation of super-enhancer-regulated neuronal function genes in the striatum of HD mice. Striatal super-enhancers display extensive H3K27 acetylation within gene bodies and drive transcription characterized by low levels of paused RNAPII. Down-regulation of gene expression is associated with diminished H3K27 acetylation and RNAPII recruitment. Striatal super-enhancers are enriched in binding motifs for Gata transcription factors, such as Gata2 regulating striatal identity genes. Thus, enhancer topography and transcription dynamics are major parameters determining the propensity of a gene to be deregulated in a neurodegenerative disease.
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| |
|
| Overall design |
Examination of H3k27ac and RNA pol II in Striatum by deep sequencing
|
| |
|
| Contributor(s) |
Merienne K |
| Citation(s) |
25784504, 28225006 |
| |
| Submission date |
Jul 18, 2014 |
| Last update date |
Mar 20, 2020 |
| Contact name |
Karine Merienne |
| E-mail(s) |
merienne@igbmc.fr
|
| Organization name |
IGBMC
|
| Street address |
1 rue Laurent Fries
|
| City |
ILLKIRCH |
| ZIP/Postal code |
67404 |
| Country |
France |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (10)
|
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| This SubSeries is part of SuperSeries: |
| GSE59572 |
Striatal genes regulated by super-enhancers and displaying low paused RNAPII are preferentially down-regulated in Huntington's disease |
|
| Relations |
| BioProject |
PRJNA255650 |
| SRA |
SRP044639 |
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