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Series GSE59828 Query DataSets for GSE59828
Status Public on Oct 07, 2014
Title Transcriptional Pause Release Is a Rate-Limiting Step for Somatic Cell Reprogramming (ChIP-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Reactivation of the pluripotency network during somatic cell reprogramming by exogenous transcription factors involves chromatin remodeling and the recruitment of RNA polymerase II (Pol II) to target loci. Here, we report that Pol II is engaged at pluripotency promoters in reprogramming but remains paused and inefficiently released. We also show that bromodomain-containing protein 4 (BRD4) stimulates productive transcriptional elongation of pluripotency genes by dissociating the pause release factor P-TEFb from an inactive complex containing HEXIM1. Consequently, BRD4 overexpression enhances reprogramming efficiency and HEXIM1 suppresses it, whereas Brd4 and Hexim1 knockdown do the opposite. We further demonstrate that the reprogramming factor KLF4 helps recruit P-TEFb to pluripotency promoters. Our work thus provides a mechanism for explaining the reactivation of pluripotency genes in reprogramming and unveils an unanticipated role for KLF4 in transcriptional pause release.
 
Overall design Pol II ChIP-seq for MEFs, ESCs and bulk populations of OSKM reprogramming intermediates at two time points.
 
Contributor(s) Liu L, Xu Y, He M, Lu L, Wang H, Esteban MA
Citation(s) 25312495
Submission date Jul 28, 2014
Last update date May 15, 2019
Contact name Minghui He
E-mail(s) hemh@genomics.cn
Organization name BGI-Shenzhen
Department Cancer Research Department
Street address Beishan Industrial Zone, Yantian District
City ShenZhen
State/province GuangDong
ZIP/Postal code 518083
Country China
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (5)
GSM1446974 MEFs_Pol II
GSM1446975 OSKM_D5_Pol II
GSM1446976 OSKM_D8_Pol II
This SubSeries is part of SuperSeries:
GSE59833 Transcriptional Pause Release Is a Rate-Limiting Step for Somatic Cell Reprogramming
Relations
BioProject PRJNA256325
SRA SRP044939

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59828_RAW.tar 390.8 Mb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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