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Series GSE59876 Query DataSets for GSE59876
Status Public on Jan 23, 2015
Title Pseudomonas aeruginosa LysR PA4203 regulator acts as a repressor of the PA4202 gene encoding a nitronate monooxygenase
Platform organisms Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1
Sample organism Pseudomonas aeruginosa PAO1
Experiment type Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary More than 7% of the Pseudomonas aeruginosa genes are encoding transcriptional regulators, many of which with unknown functions. Among them, those belonging to the LysR family are the most represented. The PA4203 gene lies upstream of the previously characterized ppgL gene (PA4204), which encodes a periplasmic gluconolactonase, which detoxifies gluconolactone by converting it to gluconate. Upstream of PA4203 and in the opposite orientation are the PA4202 gene coding for a nitronate monooxygenase and ddlA (PA4201) encoding a D-alanine alanine ligase. This genetic organization is conserved in all P. aeruginosa genomes, but not in other pseudomonads. The intergenic regions between PA4203 and ppgL, and PA4202 are very short (79 and 107 nucleotides, respectively). PA4203 is a repressor of PA4202 and of its own transcription. A chromatin immunoprecipation analysis confirmed the presence of a single PA4203 binding site between PA4202 and PA4203. Electrophoretic mobility shift assays (EMSAs) with the purified PA4203 protein and in41 gel footprinting with the 1, 10-phenanthroline-copper ion, combined with primer extension analysis to determine transcriptional startpoints allowed the identification of a LysR binding motive in the PA4202 and PA4203 intergenic region. Despite this, a transcriptome analysis revealed more genes to be affected in a PA4203 mutant, likely due to the overexpression of the nitronate monooxygenase (PA4202). Deletion of the PA4202 gene resulted in an increased sensitivity of the cells to 3- nitropropionic acid (3-NPA).
 
Overall design [expression] Two samples of a Delta PA4203 mutant compared to two samples of PAO1 wild type.
[ChIP] Two samples of PAO1-pUCP4203 compared to one sample of PAO1-wt.
 
Contributor(s) Vercammen K, Wei Q, Charlier D, Dötsch A, Häussler S, Schulz S, Salvi F, Gadda G, Spain J, Rybtke ML, Tolker-Nielsen T, Cornelis P
Citation(s) 25384477
Submission date Jul 29, 2014
Last update date Jul 06, 2016
Contact name Andreas Doetsch
E-mail(s) andreas.doetsch@mri.bund.de
Phone +497216625443
Organization name Max Rubner-Institute
Department Physiology and Biochemistry of Nutrition
Street address Haid-und-Neu-Str. 9
City Karlsruhe
ZIP/Postal code 76131
Country Germany
 
Platforms (2)
GPL84 [Pae_G1a] Affymetrix Pseudomonas aeruginosa Array
GPL19010 [PATA1w520385F] Affymetrix Pseudomonas aeruginosa PAO1 custom tiling array
Samples (7)
GSM1448868 PAO1-WT-1 [expression]
GSM1448869 PAO1-WT-2 [expression]
GSM1448870 PAO1-dPA4203-1 [expression]
Relations
BioProject PRJNA256959

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59876_RAW.tar 16.2 Mb (http)(custom) TAR (of BAR, CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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