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Series GSE59924 Query DataSets for GSE59924
Status Public on Apr 12, 2016
Title A Novel Role of Polycomb in Positive Transcription Regulation by Modulating H4K20me1
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Polycomb group (PcG) proteins were originally shown to play central roles in maintaining heritable gene suppression during the normal development of higher eukaryotes. However, recent studies in Drosophila and mammals imply a potential positive role of PcG proteins in gene transcription with unknown mechanism. Here, for the first time we report Pc-mediated, E(z)/H3K27me3-dependent positive transcription regulation of Ci and Sens, two key transcription factors involved in body plan during normal development. Further mechanistic studies showed that Pc regulates Sens expression by regulating H4K20me1 at Sens locus. More importantly, this mechanism applies to other genes involved in the developmental process. In summary, we report a novel and general transcription regulation mechanism, that is, Pc-mediated, E(z)/H3K27me3-dependent positive transcription regulation by modulating H4K20me1 during development.
Overall design H4K20me1 ChIP-seq dataset was generated in wide type Drosophila third instar larvae wing discs. RNA-seq datasets were generated for wide type, Polycomb RNAi samples.
Contributor(s) Lv X, Han Z, Chen H, Yang X, Zhang Z, Yang B, Xia Y, Lu Y, Pan C, Fu L, Han H, Mao F, Wu M, Zhou Z, Li D, Zhang L, Jiang H, Li L, Wei G, Zhao Y
Citation(s) 27002220
Submission date Jul 30, 2014
Last update date May 15, 2019
Contact name Gang Wei
Organization name CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Lab Key Laboratory of Computational Biology
Street address 320 Yue Yang Road
City Shanghai
State/province Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (4)
GSM1461743 H4K20me1 ChIP-seq WT
GSM1461744 Input ChIP-seq WT
GSM1461745 RNA-seq WT
BioProject PRJNA257070
SRA SRP045094

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Supplementary file Size Download File type/resource
GSE59924_RAW.tar 12.9 Mb (http)(custom) TAR (of BED, BW) 191.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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