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Series GSE60177 Query DataSets for GSE60177
Status Public on Jan 01, 2017
Title Zinc acquisition in Pseudomonas aeruginosa
Organism Pseudomonas aeruginosa PAO1
Experiment type Expression profiling by high throughput sequencing
Summary This study addresses the impact of zinc limitation on the opportunistic human pathogen, Pseudomonas aeruginosa. Zinc limitation was assessed in the P. aeruginosa PAO1 strain using an isogenic deletion mutant lacking the periplasmic, zinc solute-binding protein, znuA (PA5498). ZnuA delivers bound zinc to its cognate ABC transporter, ZnuBC, for import into the cytoplasm. Our transcriptional analyses revealed P. aeruginosa to possess a multitude of zinc acquisition mechanisms, each of which were highly up-regulated in the zinc-deficient znuA mutant strain. P. aeruginosa also utilized zinc-independent paralogues of zinc-dependent genes to maintain cellular function under zinc limitation. Together, these data reveal the complex transcriptional response and versatility of P. aeruginosa to zinc depletion.
Overall design The WT (zinc replete) and znuA mutant strain (zinc limited) were grown in cation-defined media to mid-log phase prior to RNA isolation and analysis.
Contributor(s) Pederick VG, Eijkelkamp BA, Begg SL, McAllister LJ, Paton JC, McDevitt CA
Citation(s) 26290475
Submission date Aug 06, 2014
Last update date May 15, 2019
Contact name Bart Eijkelkamp
Organization name University of Adelaide
Street address North Tce
City Adelaide
State/province SA
ZIP/Postal code 5000
Country Australia
Platforms (1)
GPL18782 Illumina HiSeq 2500 (Pseudomonas aeruginosa PAO1)
Samples (2)
GSM1466898 PAO1
GSM1466899 ΔznuA
BioProject PRJNA257669
SRA SRP045313

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60177_PseudomonasDESeq.txt.gz 286.0 Kb (ftp)(http) TXT
GSE60177_RAW.tar 800.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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