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Series GSE60471 Query DataSets for GSE60471
Status Public on Feb 18, 2016
Title Drosophila melanogaster high resolution developmental transcriptomic time-course
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three phyla has led us to ask if all animals pass through a universal definitive stage as a consequence of ancestral constraints on animal development. Previous work has suggested that HOX genes may comprise such a ‘zootypic’ stage, however this hypothetical stage has hitherto resisted systematic analysis. We have examined the embryonic development of ten different animals each of a fundamentally different phylum, including a segmented worm, a flatworm, a roundworm, a water bear, a fruitfly, a sea urchin, a zebrafish, a sea anemone, a sponge, and a comb jelly. For each species, we collected the embryonic transcriptomes at ~100 different developmental stages and analyzed their gene expression profiles. We found dynamic gene expression across all of the species that is structured in a stage like manner. Strikingly, we found that animal embryology contains two dominant modules of zygotic expression in terms of their protein domain composition: one involving proliferation, and a second involving differentiation. The switch between these two modules involves induction of the zootype; which in addition to homeobox containing genes, also involves Wnt and Notch signaling as well as forkhead domain transcription factors. Our results provide a systematic characterization of animal universality and identify the points of embryological constraints and flexibility.
Overall design 91 single embryo samples.
Contributor(s) Levin M, Anavy L, Yanai I
Citation(s) 26886793
Submission date Aug 18, 2014
Last update date May 15, 2019
Contact name Itai Yanai
Organization name Technion - Israel Institute of Technology
Department Biology
Lab Yanai
Street address Technion City
City Haifa
ZIP/Postal code 30200
Country Israel
Platforms (1)
GPL13304 Illumina HiSeq 2000 (Drosophila melanogaster)
Samples (91)
GSM1480615 Metazome_Drosophila_timecourse_sample_0001
GSM1480616 Metazome_Drosophila_timecourse_sample_0002
GSM1480617 Metazome_Drosophila_timecourse_sample_0003
This SubSeries is part of SuperSeries:
GSE70185 The mid-developmental transition and the evolution of animal body plans
BioProject PRJNA258368
SRA SRP045563

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