|
Status |
Public on Jul 06, 2015 |
Title |
Genome-wide mapping of promoter-enhancer interactions with HiCap [HiCap] |
Organism |
Mus musculus |
Experiment type |
Other
|
Summary |
Although the locations of promoters and enhancers have been identified in several cell types, we have yet limited information on their connectivity. We developed HiCap, which combines Hi-C with promoter sequence capture, to enable genome-wide identification of regulatory interactions with single-enhancer resolution. HiCap analyses of mouse embryonic stem cells (mESC) identified promoter-enhancer interactions predictive of gene expression change upon perturbation, opening up for genomic analyses of long-range gene regulation.
|
|
|
Overall design |
HiCap was designed by combining Hi-C with with sequence capture (for all promoters) and carried out in mouse embryonic stem cells (mESC)
|
|
|
Contributor(s) |
Abdullayev I, Ramsköld D, Sahlen P, Sandberg R |
Citation(s) |
26313521 |
|
Submission date |
Aug 18, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Ilgar Abdullayev |
E-mail(s) |
ilgar.abdullayev@licr.ki.se
|
Organization name |
Karolinska Institutet
|
Department |
Cell and Molecular Biology
|
Lab |
Rickard Sandberg's group (rickard.sandberg@ki.se)
|
Street address |
Nobels väg 3
|
City |
Stockholm |
ZIP/Postal code |
17177 |
Country |
Sweden |
|
|
Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
Samples (6)
|
|
This SubSeries is part of SuperSeries: |
GSE60495 |
Genome-wide mapping of promoter-enhancer interactions with HiCap |
|
Relations |
BioProject |
PRJNA258410 |
SRA |
SRP045580 |