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Series GSE61031 Query DataSets for GSE61031
Status Public on Nov 17, 2014
Title Dicer WT/KO MSC RNA-Seq [polyA RNA]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary RNA-Seq performed on Dicer KO and WT murine mesenchymal stem cells
MicroRNAs (miRNAs) are small non-coding RNAs that regulates development and disease but induce only moderate repression of directs mRNA targets, suggesting that they coordinate with other modes ofs cellular regulation to effect large changes in gene expression. Ins this work we decouple direct effects of global miRNA loss froms transcriptional changes downstream in a pair of isogenic murines fibroblast cell lines with and without Dicer expression. Wes demonstrate how effects on direct miRNA targets are amplified bys transcription machinery through the construction of a network models that identifies specific transcription factors that cause changes ins mRNA expression upon Dicer loss. Through transcription factors over-expression, we delineate miRNA-mediated transcriptional programss and identify miRNA-mediated coherent and incoherent feed-forwards loops, suggesting a functional role of the interaction between miRNAss and transcription factors. In total, our results indicate thats miRNAs tightly control transcription factors within a denses interconnected network to modulate gene expression.
Overall design Examination of mature mRNA expression changes in adult mesenchymal stem cells (immortalized monoclonal lines of murine MSCs) with and without Dicer (WT: Dicer f/f, KO: Dicer -/-).
Contributor(s) Gosline SJ
Citation(s) 25449132
BioProject PRJNA257487
Submission date Sep 03, 2014
Last update date May 15, 2019
Contact name Sara J Gosline
Organization name MIT
Department Biological Engineering
Lab Fraenkel
Street address 77 Massachusetts Ave
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1495725 Dicer KO Fibroblasts
GSM1495726 Dicer WT Fibroblasts
SRA SRP045262

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61031_DE_polyaWT_vs_polyaKO.txt.gz 990.0 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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