|
| Status |
Public on Nov 17, 2014 |
| Title |
Change in H3K4me3 in Suz12(Bgal/Bgal) ESCs, and over Spinal Motor Neuron differentiation (ChIP-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Suz12(Bgal/Bgal) ESCs express a truncated form of Suz12 fused to Beta-galactosidase. These cells maintain a reduced level of H3K27me3 despite this mutation to a core component of PRC2, unlike Eed-/- ESCs whose H3K27me3 is ablated. This data shows the concomitant changes in H3K4me3 levels in these cells.
|
| |
|
| Overall design |
An ESC line mutant in Suz12, a core component of Polycomb Repressive Complex 2, was assessed for H3K4me3 by ChIP-seq, as compared to a wild type ESC line, as well as both lines subjected to in vitro differentiation down the Spinal Motor Neuron pathway.
|
| |
|
| Contributor(s) |
Thornton SR, Boyer LA |
| Citation(s) |
25333635 |
| |
| Submission date |
Sep 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Laurie A Boyer |
| E-mail(s) |
lboyer@mit.edu
|
| Phone |
617 324-3335
|
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Biology
|
| Lab |
Boyer
|
| Street address |
77 Massachusetts Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (4)
|
| GSM1500752 |
H3K4me3 ChIP-seq in WT E14 ESCs |
| GSM1500753 |
H3K4me3 ChIP-seq in E14 ESC-derived Spinal Motor Neuron lineage cells |
| GSM1500754 |
H3K4me3 ChIP-seq in Suz12GT (aka Suz12(Bgal/Bgal)) ESCs |
| GSM1500755 |
H3K4me3 ChIP-seq in Suz12GT (aka Suz12(Bgal/Bgal)) ESC-derived Spinal Motor Neuron lineage cells |
|
| This SubSeries is part of SuperSeries: |
| GSE53508 |
PRC2 coordinates lineage fidelity and DNA methylation during ESC differentiation |
|
| Relations |
| BioProject |
PRJNA260591 |
| SRA |
SRP046724 |
Less...
More...