|
| Status |
Public on Aug 24, 2015 |
| Title |
Stationary phase stimulon and CspC regulon |
| Platform organism |
Caulobacter vibrioides CB15 |
| Sample organism |
Caulobacter vibrioides NA1000 |
| Experiment type |
Expression profiling by array
|
| Summary |
The cold shock proteins belong to a family of RNA binding proteins presenting a highly conserved domain, called cold shock domain (CSD). They are involved in various cellular processes, including adaptation to low temperature, nutritional stress, cell growth and stationary phase. Here we investigate the role of CspC in C. crescentus stationary phase and the molecular mechanisms underlying gene regulation by this protein. A global transcriptional profiling experiment comparing cspC and the wild type strain both at exponential and stationary phases was carried out. The results showed that the absence of cspC affected the transcription of 20 genes at exponential phase and 65 genes at stationary phase. Genes encoding enzymes of the glyoxylate cycle were severely downregulated in the mutant at stationary phase. The stationary phase-induced RNA binding protein CspC has an important role in gene expression at this phase. It is required for the expression of the essential gene sciP, the ECF sigma factor sigU, as well as of the genes for the glyoxylate cycle enzymes and for oxidative stress response.
|
| |
|
| Overall design |
Two and three replicates were performed to determine the stationary phase stimulon and CspC regulon, respectively. Each replicate was conducted with an independent biological sample.
|
| |
|
| Contributor(s) |
Santos JS, da Silva CA, Balhesteros H, Lourenço RF, Marques MV |
| Citation(s) |
26311251 |
| |
| Submission date |
Sep 24, 2014 |
| Last update date |
Jun 07, 2019 |
| Contact name |
Juliana da Silva Santos |
| E-mail(s) |
s.juliana.s@usp.br
|
| Phone |
551130917299
|
| Organization name |
Institute of Biomedical Sciences from University of São Paulo
|
| Department |
Microbiology
|
| Lab |
222
|
| Street address |
Avenida Professor Lineu Prestes, 1374
|
| City |
São Paulo |
| State/province |
SP |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
| |
|
| Platforms (1) |
| GPL10469 |
MIT Agilent Caulobacter 45K Array |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA261960 |
Less...
More...