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Series GSE61726 Query DataSets for GSE61726
Status Public on Aug 24, 2015
Title Stationary phase stimulon and CspC regulon
Platform organism Caulobacter vibrioides CB15
Sample organism Caulobacter vibrioides NA1000
Experiment type Expression profiling by array
Summary The cold shock proteins belong to a family of RNA binding proteins presenting a highly conserved domain, called cold shock domain (CSD). They are involved in various cellular processes, including adaptation to low temperature, nutritional stress, cell growth and stationary phase. Here we investigate the role of CspC in C. crescentus stationary phase and the molecular mechanisms underlying gene regulation by this protein. A global transcriptional profiling experiment comparing cspC and the wild type strain both at exponential and stationary phases was carried out. The results showed that the absence of cspC affected the transcription of 20 genes at exponential phase and 65 genes at stationary phase. Genes encoding enzymes of the glyoxylate cycle were severely downregulated in the mutant at stationary phase. The stationary phase-induced RNA binding protein CspC has an important role in gene expression at this phase. It is required for the expression of the essential gene sciP, the ECF sigma factor sigU, as well as of the genes for the glyoxylate cycle enzymes and for oxidative stress response.
Overall design Two and three replicates were performed to determine the stationary phase stimulon and CspC regulon, respectively. Each replicate was conducted with an independent biological sample.
Contributor(s) Santos JS, da Silva CA, Balhesteros H, Lourenço RF, Marques MV
Citation(s) 26311251
Submission date Sep 24, 2014
Last update date Jun 07, 2019
Contact name Juliana da Silva Santos
Phone 551130917299
Organization name Institute of Biomedical Sciences from University of São Paulo
Department Microbiology
Lab 222
Street address Avenida Professor Lineu Prestes, 1374
City São Paulo
State/province SP
ZIP/Postal code 05508000
Country Brazil
Platforms (1)
GPL10469 MIT Agilent Caulobacter 45K Array
Samples (8)
GSM1652139 ΔcspC x WT_log phase_rep1
GSM1652140 ΔcspC x WT_log phase_rep2
GSM1652141 ΔcspC x WT_log phase_rep3
BioProject PRJNA261960

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61726_RAW.tar 77.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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