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Series GSE61752 Query DataSets for GSE61752
Status Public on Aug 01, 2016
Title Identification of cell signaling pathway associated miRNAs in cancer cells using a systems biological approach: potent RNAi response in cancer using novel 2’-OMe-phosphorodithioated modified siRNAs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MicroRNAs (miRNAs) are single-stranded, non-coding RNA molecules and on average only 22 nucleotides long that regulate gene expression at the post-transcriptional level. Target predictions of miRNA based on sequence are limited due to too many candidates resulting in high false positives. We generated the Association score matrix and miRNA-pathway association map. To validate our approach, we used miR-500 as an example to investigate the hypothetical target regulation mechanisms. We confirmed in-vitro that mir-500 highly is associated with ERK signaling pathway in breast cancer cells. Furthermore, we found PPFIA1 might be one of direct targets of miR-500 to regulate activating the ERK pathway. In conclusion, we developed a model system to systematically predict potential miRNA functional targets which play critical roles in cell signaling pathways from the NCI-60 genomic and proteomic data at whole genomic scale.
 
Overall design Two groups of samples are included: 1. siControl treated control-miRNA and 2. mimic miR-500 treated two cells: MCF7 and MDA-MB-231. Gene expression profiles of mimic miR-500 treated cells were compared to that of control treated cells.
 
Contributor(s) Kim S, Lee J
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Submission date Sep 25, 2014
Last update date Aug 13, 2018
Contact name Sangbae Kim
E-mail(s) sangbkim22@gmail.com
Organization name Baylor Colleage of Medicine
Department HGSC
Street address 1 Baylor plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (12)
GSM1513369 Control.1_MB231
GSM1513370 Control.2_MB231
GSM1513371 Control.3_MB231
Relations
BioProject PRJNA262037

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE61752_RAW.tar 26.2 Mb (http)(custom) TAR
GSE61752_rawData_cont.vs.p500.v2.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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