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| Status |
Public on Oct 15, 2014 |
| Title |
A Novel Population of Human Cardiac Resident Mesenchymal Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We describe a novel population of human adult cardiac resident stem cells (CRSCs), which are positive for W8B2 antigen, originating from human adult atrial appendages. W8B2+ CRSCs exhibit a spindle-shaped morphology, are clonogenic and able to self-renew. W8B2+ CRSCs show high expression of mesenchymal but not hematopoietic nor endothelial markers. W8B2+ CRSCs expressed GATA4, HAND2, and TBX5, but not C-KIT, SCA-1, NKX2.5, PDGFRα, ISL1 or Wilm’s tumor gene-1 (WT1). W8B2+ CRSCs can differentiate into the cardiovascular lineages and secrete various cytokines.
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| Overall design |
Comparative RNA sequencing was performed using W8B2+ cell from human atrial appendages (passage 2 from 3 different patients), c-Kit+ cell from human atrial appendages (passage 2 from 3 different patients) and W8B2+ cell from bone marrow (passage 3 from 2 different patients, from PromoCell, Heidelberg, Germany).
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| Contributor(s) |
Lim SY, Hewitt AW |
| Citation(s) |
26184084 |
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| Submission date |
Oct 14, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Alex Hewitt |
| E-mail(s) |
hewitt.alex@gmail.com
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| Organization name |
University of Melbourne
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| Lab |
CERA
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| Street address |
32 Gisborne St
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| City |
East Melbourne |
| ZIP/Postal code |
3002 |
| Country |
Australia |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA263766 |
| SRA |
SRP048889 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62288_RAW.tar |
56.3 Mb |
(http)(custom) |
TAR (of GTF) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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