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| Status |
Public on Nov 14, 2006 |
| Title |
Identification of the gene transcription mediated by TGF-β-Smad2/3-Smad4 signaling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
TGF-β signaling is known to be very much dependent on the formation of Smad2/3-Smad4 transcription regulatory complexes. However, the signaling functions of Smad2/3-Smad4 in TGF-β-induced responses are obscure as TGF-β also initiates a number of other signaling pathways. In this study, we systematically assessed the contribution of TGF-β-Smad2/3-Smad4 signaling to target gene transcription. Individual Smads were selectively knocked down in Hep3B cells by stable RNA interference (RNAi). We identified TGF-β-responsive genes using genome-wide oligonucleotide microarrays and confirmed their dependency on Smad2, Smad3 or Smad4 by the combination of RNAi and microarray assay. The major finding from our microarray analysis was that of the 2039 target genes seen to be regulated via TGF-β induction, 190 were differentially transcriptionally controlled by Smad2-Smad4 and Smad3-Smad4 signaling and the latter control mechanism appeared to be functionally more important. We also found evidence of competition between Smad2 and Smad3 for their activation when controlling the transcription of target genes.
Keywords: cell type comparison
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| Overall design |
Large-scale microarray analysis was employed for comparison between the responses of wild-type Hep3B cell line and each knockdown cell line, with and without TGF-β induction, to identify target genes and any false positive genes.
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| Contributor(s) |
Jian Y, Liang Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 07, 2006 |
| Last update date |
Jan 18, 2013 |
| Contact name |
yu jian |
| E-mail(s) |
yujian@capitalbio.com
|
| Phone |
(86)-10-80726868
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| Fax |
(86)-10-62773059
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| Organization name |
Tsinghua University
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| Department |
Medical Systems Biology Research Center
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| Street address |
Qinghuayuan
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
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| Platforms (1) |
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| Samples (7)
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| GSM143690 |
Identification of TGFb-responsive genes in Hep3B cells |
| GSM143693 |
Identification of Smad2-dependent genes in Hep3B cells |
| GSM143842 |
Identification of Smad3-dependent genes in Hep3B cells |
| GSM143843 |
Identification of Smad4-dependent genes in Hep3B cells |
| GSM143845 |
Identification of the off-target genes induced by Smad2-RNAi |
| GSM143846 |
Identification of the off-target genes induced by Smad3-RNAi |
| GSM143847 |
Identification of the off-target genes induced by Smad4-RNAi |
|
| Relations |
| BioProject |
PRJNA97513 |
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