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Series GSE63880 Query DataSets for GSE63880
Status Public on May 29, 2015
Title Modeling Clostridium difficile infections
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Clostridium difficile (Cd) is a gram-positive, spore-forming bacterium and the primary cause of nosocomial diarrhea and pseudomembranous colitis. The pathogenicity of Cd has been linked to its production of TcdA and TcdB. While they cause fluid secretion, inflammation, and colonic damage, their respective and synergistic roles have been difficult to ascertain. In infection animal model, TcdB has been demonstrated to be a key virulence factor, and TcdB causes obvious damage in human and porcine colonic explants. Using the colonic epithelia derived from cloned colonic stem cells, we have developed a model to test the response to TcdB. Epithelia generated in air-liquid interface cultures from cloned transverse colon stem cells were challenged with TcdB at different concentrations and durations.
 
Overall design Colonic stem cells were differentiated in air-liquid interface cultures and treated with C. difficile toxin B at different doses (100 pM, 250 pM, and 500 pM) and times (8 hrs, 16 hrs and 24 hrs). At appropriate time points, membranes with ALI structure were collected for histology and microarray analysis.
 
Contributor(s) Wang X, Yamamoto Y, Wilson L, Zhang T, Chevalier B, McKeon F, Xian W
Citation(s) 26040716
Submission date Dec 04, 2014
Last update date Feb 18, 2019
Contact name Frank McKeon
E-mail(s) mckeon.xian@gmail.com
Phone 8608372486
Organization name University of Houston
Department Biology and Biochemistry
Street address 3455 Cullen Blvd
City Houston
State/province TX
ZIP/Postal code 77004
Country USA
 
Platforms (1)
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (20)
GSM1558880 TcdB_100pM_16hr_rep1
GSM1558881 TcdB_100pM_16hr_rep2
GSM1558882 TcdB_100pM_24hr_rep1
This SubSeries is part of SuperSeries:
GSE66749 Cloning and Variation of Ground State Intestinal Stem Cells Super [expression & SNP studies]
Relations
BioProject PRJNA269311

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63880_RAW.tar 391.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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