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Series GSE64540 Query DataSets for GSE64540
Status Public on Jun 22, 2017
Title Transient Hes5 activity instructs mesodermal cells toward a cardiac fate
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Notch signaling plays a role in specifying a cardiac fate but the downstream effectors remain unknown. In this study we implicate the Notch downstream effector HES5 in cardiogenesis. We show transient Hes5 expression in early mesoderm of gastrulating embryos and demonstrate, by loss and gain-of-function experiments in mouse embryonic stem cells, that HES5 favors cardiac over primitive erythroid fate. Hes5 overexpression promotes upregulation of the cardiac gene Isl1, while the hematopoietic regulator Scl is downregulated. Moreover, whereas a pulse of Hes5 instructs cardiac commitment, sustained expression after lineage specification impairs progression of differentiation to contracting cardiomyocytes. These findings establish a role for HES5 in cardiogenesis and provide insights into the early cardiac molecular network.
Overall design ChIP-Seq was performed on the indicated cell lines.
Contributor(s) Freire AG, Waghray A, Pinto-do-Ó P, Lemischka IR
Citation(s) 28648899
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 GM078465 Global & Systems Level Analyses of Cell Fate Regulation MOUNT SINAI SCHOOL OF MEDICINE IHOR R LEMISCHKA
Submission date Dec 29, 2014
Last update date May 15, 2019
Contact name avinash waghray
Organization name Massachussets General Hospital
Department Center for Regenerative Medicine
Lab Rajagopal
Street address 185 Cambridge Street, CPZN Room 4254A
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (2)
GSM1573758 D3.75 Bry+ cells 48h-Dox ChIPSeq
GSM1573759 D3.75 Bry+ cells 48h+Dox ChIPSeq
BioProject PRJNA271275
SRA SRP051587

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Supplementary file Size Download File type/resource
GSE64540_RAW.tar 221.6 Mb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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