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Series GSE64601 Query DataSets for GSE64601
Status Public on Aug 19, 2015
Title MacroH2A1 ChIP-seq from H-RasV12 expressing senescent IMR90 human primary lung fibroblasts
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Oncogene induced senescence (OIS) is a tumor suppressive mechanism typified by stable proliferative arrest, a persistent DNA damage response and the senescent-associated secretory phenotype (SASP). MacroH2A1, a tumor suppressive histone variant, is upregulated during OIS. Using ChIP-seq, we found that macroH2A1 undergoes dramatic relocalization during OIS. SASP genes are enriched in macroH2A1-containing chromatin and macroH2A1 is a critical component of the positive feedback loop that maintains SASP expression. Endoplasmic reticulum (ER) stress, a feature of OIS that requires macroH2A1, leads to ATM activation. ER stress triggers a negative feedback loop reducing SASP expression by causing the ATM-dependent removal of macroH2A1 from SASP genes. MacroH2A1 represents a critical control point in the regulation of SASP expression during OIS by We demonstrate that SASP gene expression is regulated by the combined actions of a positive feedback loop that requires macroH2A1 and a negative feedback loop where ER stress leads to ATM activation critical for the removal of macroH2A1 from SASP genes and consequently their repression.
Overall design macroH2A1 ChIP and corresponding input samples from H-RasV12 OIS IMR90 cells were sequenced
Contributor(s) Gamble MJ, Chen H
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Submission date Dec 31, 2014
Last update date May 15, 2019
Contact name Matthew Jon Gamble
Organization name Albert Einstein College of Medicine
Department Molecular Pharmacology
Lab Gamble Lab
Street address 1300 Morris Park Ave / Golding 203
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM1575698 macroH2A1 OIS ChIP replicate 1
GSM1575699 OIS input replicate 1
BioProject PRJNA271454
SRA SRP051655

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Supplementary file Size Download File type/resource
GSE64601_RAW.tar 1.7 Mb (http)(custom) TAR (of BEDGRAPH, GAPPEDPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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