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Series GSE65427 Query DataSets for GSE65427
Status Public on Nov 04, 2015
Title Genome-wide studies identify a novel interplay between AML1 and AML1/ETO in t(8;21) acute myeloid leukemia
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The AML1/ETO fusion protein is essential to the development of acute myeloid leukemia (AML), and is well recognized for its dominant-negative effect on the co-existing wild-type protein AML1. However, the involvement of wild-type AML1 in AML1/ETO-driven leukemogenesis remains elusive. Through chromatin immunoprecipitation sequencing, computational analysis plus a series of experimental validations, we report here that AML1 is able to orchestrate the expression of AML1/ETO targets regardless of being activated or repressed, via forming a complex with AML1/ETO and via recruiting the cofactor.
Overall design 4 ChIP-seq assays were used to identify the high confidence binding regions of AML1-ETO and AML1 in t(8;21) AML Kasumi-1 cell lines. The anti-AML1 (N20) antibody targets the N-terminus of AML1 and recognizes both AML1 and AML1/ETO; the anti-AML1 (C19) antibody targets the C-terminus of AML1 and recognizes AML1 but not AML1/ETO; the anti-ETO (C20) antibody targets the C-terminus of ETO and specifically recognizes AML1/ETO. 2 ChIP-seq assays were used to identify the binding regions of AML1 in human macrophage U937 cell lines. And the total input was used as control.
Contributor(s) Wang K, Li Y, Wang H
Citation(s) 26546158
Submission date Jan 29, 2015
Last update date May 15, 2019
Contact name Huanwei Wang
Phone +86-18817821254
Organization name Rui-Jin Hospital affiliated to Shanghai Jiao Tong University School of Medicine (RJH-SJTU-SM)
Department Shanghai Institute of Hematology
Street address 197 Ruijin Rd II
City shanghai
State/province shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (6)
GSM1595963 Ka.Con.AML1-C19
GSM1595964 Ka.Con.AML1-N20
GSM1595965 Ka.Con.ETO
BioProject PRJNA273981
SRA SRP052956

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Supplementary file Size Download File type/resource
GSE65427_RAW.tar 1.0 Gb (http)(custom) TAR (of BED, BIGWIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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