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Status |
Public on Dec 18, 2006 |
Title |
Expression profiling of A. thaliana wild type Columbia-0 and mutant gh3.5-1D in response to pathogen Pst DC3000(avrRpt2) |
Organism |
Arabidopsis thaliana |
Experiment type |
Expression profiling by array
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Summary |
To reveal the underlying molecular mechanism of GH3.5 action in modulating the SA and auxin pathways, we performed transcriptional profiling of gh3.5-1D plants after infection with or without Pst DC3000(avrRpt2) on a global scale using the Affymetrix Arabidopsis ATH1 GeneChip Keywords: Pathogen-response
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Overall design |
Each 3 leaves of plants of 5-week-old wild type Columbia-0 and mutant gh3.5-1D (heterozygous) were inoculated with Pst DC3000(avrRpt2)at 105 cfu mL-1. Leaves were harvested at 0 h (uninoculated) and 48 hours after inoculation. Three biological repeats were performed on Columbia-0 (wild-type, Col-0) and gh3.5-1D (mutant) after infection with or without Pst DC3000(avrRpt2), respectively.
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Contributor(s) |
Zhang Z, Li Q, Li Z, Staswick PE, Wang M, Zhu Y, He Z |
Citation(s) |
17704230 |
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Submission date |
Dec 18, 2006 |
Last update date |
Aug 28, 2018 |
Contact name |
Li Qun |
E-mail(s) |
qli@sibs.ac.cn
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Phone |
+86 21 54924122
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Organization name |
Institute of plant physiology & ecology, SIBS, CAS
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Department |
National Key Laboratory of Plant Molecular Genetics
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Street address |
300 Fenglin Road
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City |
Shanghai |
State/province |
Shanghai |
ZIP/Postal code |
200032 |
Country |
China |
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Platforms (1) |
GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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Samples (12)
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GSM151694 |
Col-0 uninoculated, biological replicate 1 |
GSM151695 |
Col-0 uninoculated, biological replicate 2 |
GSM151696 |
Col-0 uninoculated, biological replicate 3 |
GSM151697 |
gh3.5-1D uninoculated, biological replicate 1 |
GSM151698 |
gh3.5-1D uninoculated, biological replicate 2 |
GSM151699 |
gh3.5-1D uninoculated, biological replicate 3 |
GSM151700 |
Col-0 48 hpi, biological replicate 1 |
GSM151701 |
Col-0 48 hpi, biological replicate 2 |
GSM151702 |
Col-0 48 hpi, biological replicate 3 |
GSM151703 |
gh3.5-1D 48 hpi, biological replicate 1 |
GSM151704 |
gh3.5-1D 48 hpi, biological replicate 2 |
GSM151705 |
gh3.5-1D 48 hpi, biological replicate 3 |
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Relations |
Affiliated with |
GSE69995 |
BioProject |
PRJNA98769 |
Supplementary file |
Size |
Download |
File type/resource |
GSE6556_RAW.tar |
37.1 Mb |
(http)(custom) |
TAR (of CEL) |
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