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Series GSE65883 Query DataSets for GSE65883
Status Public on Dec 31, 2018
Title Genome-wide association of Sus1 with RNAPII and tRNA genes depends on the distinctive SAGA subunits Spt8 and Spt7
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by genome tiling array
Summary We have analyzed the global effect of the conserved transcription-mRNA export factor Sus1 on transcription and its association with chromatin. We used genomic run-on experiments to show that Sus has a broad impact on the stability of most RNA polymerase II-transcribed genes. Genome association of Sus1 by the chromatin immunoprecipitation technique showed that Sus1 was widely distributed throughout coding regions, tending to accumulate towards the 3’ ends of highly transcribed transcription factor IID (TFIID) and Spt-Ada-Gcn5 acetyltransferase (SAGA) dependent genes. This accumulation depends on growth conditions, the transcriptional rate and whether the genes are TFIID- or SAGA-regulated. Validation of Sus1 occupancy data also revealed that Sus1 appears at tRNAs. Concomitantly, deletion of SUS1 leads to tRNA overexpression. In addition, we discovered that distinctive SAGA subunits Spt8 and Spt7 play a key role in Sus1 enrichment at the 3’ ends of SAGA-regulated genes upon temperature shift and at tRNAs. Thus, our study identifies the molecular mechanisms by which a SAGA factor is recruited genome-wide, and provides evidence of a more general role for this conserved coactivator in eukaryotic transcription.
 
Overall design 12 samples, 4 strains (WT, spt8Δ, spt7-1180, spt8Δspt7-1180), 3 replicates per strain
 
Contributor(s) García-Molinero V, Furió-Tarí P, Antúnez O, Han KH, Vinayachandran V, Reja R, Pugh F, Pérez-Ortín JE, Conesa A, Rodríguez-Navarro S
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Submission date Feb 12, 2015
Last update date Jan 01, 2019
Contact name VARINIA GARCIA-MOLINERO
Organization name CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE
Street address EDUARDO PRIMO YÚFERA, 3
City VALENCIA
ZIP/Postal code 46012
Country Spain
 
Platforms (1)
GPL19774 [Scerevisiae_tlg] Affymetrix Saccharomyces cerevisiae tiling array
Samples (12)
GSM1608243 WT_rep1
GSM1608244 WT_rep2
GSM1608245 WT_rep3
Relations
BioProject PRJNA275304

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65883_RAW.tar 351.2 Mb (http)(custom) TAR (of CEL)
GSE65883_Spt7-1180_vs_WT_F_signal.bar.gz 6.6 Mb (ftp)(http) BAR
GSE65883_Spt7-1180_vs_WT_R_signal.bar.gz 6.6 Mb (ftp)(http) BAR
GSE65883_spt8d_Spt7-1180_vs_WT_F_signal.bar.gz 6.8 Mb (ftp)(http) BAR
GSE65883_spt8d_Spt7-1180_vs_WT_R_signal.bar.gz 6.8 Mb (ftp)(http) BAR
GSE65883_spt8d_vs_WT_F_signal.bar.gz 6.7 Mb (ftp)(http) BAR
GSE65883_spt8d_vs_WT_R_signal.bar.gz 6.7 Mb (ftp)(http) BAR
Processed data are available on Series record

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