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Series GSE65918 Query DataSets for GSE65918
Status Public on Sep 17, 2015
Title Oct1 and OCA-B are Selectively Required for CD4 Memory T Cell Formation and Function (ChIP-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Epigenetic changes are crucial for the generation of immunological memory. Failure to generate or maintain these changes will result in poor memory responses. Similarly, augmenting or stabilizing the correct epigenetic states offers a potential method of enhancing immune memory. Yet the transcription factors that regulate these processes are poorly defined, as are the target genes they control and they chromatin-modifying complexes they recruit. Using model pathogens and three different mouse models, we find that the widely expressed transcription factor Oct1 and its cofactor OCA-B are selectively required for the in vivo generation of functional CD4 memory. In vitro, both proteins are required to maintain a poised state at the Il2 target locus in resting but previously stimulated CD4 T cells, and to generate robust Il2 expression upon restimulation. Gene expression profiling indicates that OCA-B is also required for the robust re-expression of multiple other targets including Ifng and Il17a. ChIPseq identify multiple differentially expressed direct targets. We identify an underlying mechanism involving OCA-B recruitment of the histone lysine demethylase Jmjd1a to targets such as Il2 and Ifng. The findings pinpoint Oct1 and OCA-B as unanticipated mediators of CD4 T cell memory.
 
Overall design Examination of transcription factor occupancy in CD4 T cells upon rest and restimulation.
 
Contributor(s) Shakya A, Goren A, Shalek A, German C, Snook J, Kuchroo V, Yosef N, Chan R, Williams M, Tantin D
Citation(s) 26481684
Submission date Feb 13, 2015
Last update date May 15, 2019
Contact name Dean Tantin
E-mail(s) dean.tantin@path.utah.edu
Phone (801) 587-3035
Organization name University of Utah
Department Department of Pathology
Street address 15 North Medical Drive East
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (6)
GSM1611105 Rest_Oct1_ChIPSeq
GSM1611106 Rest_OcaB_ChIPSeq
GSM1611107 Rest_Input_ChIPSeq
This SubSeries is part of SuperSeries:
GSE65925 Oct1 and OCA-B are Selectively Required for CD4 Memory T Cell Formation and Function
Relations
BioProject PRJNA275419
SRA SRP055021

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65918_Common_intersect.bed.gz 46.8 Kb (ftp)(http) BED
GSE65918_RAW.tar 1.6 Gb (http)(custom) TAR (of BED, TDF)
GSE65918_Shakya_Table_S2.xlsx 190.8 Kb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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