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| Status |
Public on Aug 03, 2015 |
| Title |
Effect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos |
| Platform organism |
Bos taurus |
| Sample organism |
Bos indicus |
| Experiment type |
Expression profiling by array
|
| Summary |
Changes in gene expression induced by the Cryotop vitrification procedure in bovine blastocysts using Agilent EmbryoGENE microarray slides. Bovine in vitro-produced embryos at the blastocyst stage (144 to 156 hours post insemination) were vitrified using the Cryotop system and compared with non-vitrified (control) embryos. After vitrification, the embryos were warmed and cultured for an additional 4 hours. Embryos that re-expanded or developed to the expanded blastocyst stage were used for microarray analysis.
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| |
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| Overall design |
Four pools of vitrified embryos were hybridized against four pools of control embryos in a dye-swap design.
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| |
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| Contributor(s) |
Leme LO, Dufort I, Sprícigo JF, Braga TF, Sirard M, Franco MM, Dode MA |
| Citation(s) |
26553569 |
| |
| Submission date |
Feb 16, 2015 |
| Last update date |
Nov 12, 2018 |
| Contact name |
Marc-André Sirard |
| E-mail(s) |
Marc-Andre.Sirard@fsaa.ulaval.ca
|
| Organization name |
Université Laval
|
| Department |
Sciences Animales
|
| Street address |
Offfice 2732, 2440 Hochelaga Blvd.
|
| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
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| Platforms (1) |
| GPL13226 |
Agilent-028298 Embryogene Bovine 45K microarray |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA275547 |
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