NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE6640 Query DataSets for GSE6640
Status Public on Nov 08, 2007
Title Profiling of sex-biased expression in Drosophila species
Organisms Drosophila ananassae; Drosophila melanogaster; Drosophila mojavensis; Drosophila pseudoobscura; Drosophila simulans; Drosophila virilis; Drosophila yakuba
Experiment type Expression profiling by genome tiling array
Summary Extensive sex-biased expression has been seen in multiple surveys D. melanogaster. We were interested in broadly sampling sex-biased expression of orthologs and species- or lineage-specific genes in the Drosophila genus. To appropriately assay gene expression in multiple species, we used custom microarrays designed against each of six species that broadly sample the phylogenetic space represented by the newly completed genomes (D. simulans, D. yakuba, D. ananassae, D. pseudoobscura, D. virilis and D. mojavensis) and an array designed against D. melanogaster to determine the overall patterns of sex-biased expression in those species and their chromosome linkage.
Keywords: other
 
Overall design PolyA+ mRNA in duplicate were isolated from 5-7 day old adult females and males of seven drosophila species. Male and female mRNA was labeled by Cy3 or Cy5 dye separately and hybridized simultaneously to the appropriate species-specific arrays. We performed at least four two-channel hybridizations for each species including two biological replicates and dye-swaps for each of them. Only single channels which passed stringent pre-processing quality control were used. A total of 67 channels of data were used in the final analysis.
 
Contributor(s) Sturgill D, Zhang Y, Oliver B
Citation(s) 17994089
Submission date Jan 04, 2007
Last update date Sep 19, 2012
Contact name Brian Oliver
E-mail(s) briano@nih.gov
Phone 301-204-9463
Organization name NIDDK, NIH
Department LBG
Lab Developmental Genomics
Street address 50 South Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (7)
GPL4628 Nimblegen Dana expression custom array/2005-06-03_D_ananassae_50mer
GPL4629 Nimblegen Drosophila melanogaster expression array/2005-10-17_Dmel4_60mer_exp
GPL4630 Nimblegen Dmoj expression custom array/2005-06-03_D_mojavensis_50mer
Samples (72)
GSM149655 Dsim_male_rep1
GSM149656 Dsim_male_rep2
GSM149657 Dsim_male_rep3
Relations
BioProject PRJNA98833

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6640_RAW.tar 604.3 Mb (http)(custom) TAR (of FTR)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap