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Series GSE66504 Query DataSets for GSE66504
Status Public on May 05, 2015
Title Identification of N6-adenine methylation in C. elegans DNA
Organism Caenorhabditis elegans
Experiment type Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary In mammalian cells, DNA methylation on the 5th position of cytosine (5mC) plays an important role as an epigenetic mark. However, DNA methylation was considered to be absent in C. elegans because of the lack of detectable 5mC as well as homologs of the cytosine DNA methyltransferases. Here, using multiple approaches, we demonstrate the presence of adenine N6-methylation (6mA) in C. elegans DNA. We further demonstrate that this modification increases trans-generationally in a paradigm of epigenetic inheritance. Importantly, we identify a DNA demethylase, NMAD-1, and a potential DNA methyltransferase, DAMT-1, which regulate 6mA levels and crosstalk between methylation of histone H3K4me2 and 6mA, and control the epigenetic inheritance of phenotypes associated with the loss of the H3K4me2 demethylase spr-5. Together, these data identify a novel DNA modification in C. elegans and raise the exciting possibility that 6mA may be a carrier of heritable epigenetic information in eukaryotes.
Overall design SMRT-sequencing for a mixed cell population of wildtype worms
6mA ChIP-Seq for a mixed cell population of wildtype worms
Contributor(s) Gu L, Greer EL, Blanco MA, Sendinc E, Liu J, Aristizabal-Corrales D, Hsu C, Aravind L, He C, Shi Y
Citation(s) 25936839, 31159718
Submission date Mar 04, 2015
Last update date Jun 16, 2019
Contact name Lei Gu
Organization name Max Planck Institute for heart and lung research
Street address parkstreet 1
City Bad Nauheim
State/province MASSACHUSETTS
ZIP/Postal code 61231
Country Germany
Platforms (2)
GPL18245 Illumina HiSeq 2500 (Caenorhabditis elegans)
GPL19919 PacBio RS II (Caenorhabditis elegans)
Samples (3)
GSM1637798 SMRT-sequencing
GSM1688840 ChIP-Seq
GSM1688841 ChIP-Seq (input)
BioProject PRJNA278967
SRA SRP056370

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Supplementary file Size Download File type/resource
GSE66504_RAW.tar 341.4 Mb (http)(custom) TAR (of BW, GFF, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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