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Series GSE66735 Query DataSets for GSE66735
Status Public on May 07, 2015
Title Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity for Pluripotency and Reprogramming [ChIP-Seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for Oct4, Sox2, and Nanog in the enhanceosome assembly and express enhancer RNAs (eRNAs). We sought to dissect the molecular control mechanism of SE activity and eRNA transcription for pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, a key pluripotency and reprogramming factor that guides the ESC-specific enhanceosome assembly and orchestrates the hierarchical transcriptional activation during the final stage of reprogramming, we discovered Tex10 as a novel pluripotency factor that is evolutionally conserved and functionally significant in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation of SEs. Our study sheds new light on epigenetic control of SE activity for cell fate determination.
Overall design Genome binding/occupancy profiling of Tex10 was performed in mouse embryonic stem cells by ChIP sequencing.
Contributor(s) Ding J, Huang X, Wang J
Citation(s) 25936917
Submission date Mar 10, 2015
Last update date May 15, 2019
Contact name Jianlong Wang
Organization name Columbia University Irving Medical Center
Department Department of Medicine
Lab Jianlong Wang
Street address 650 W. 168th St.
City New York
State/province New York
ZIP/Postal code 10032
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (3)
GSM1631192 mESC_ChIPseq_Flag(Tex10)
GSM1631193 mESC_ChIPseq_IgG
GSM1631194 mESC_ChIPseq_Input
This SubSeries is part of SuperSeries:
GSE66736 Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity for Pluripotency and Reprogramming
BioProject PRJNA277842
SRA SRP056039

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66735_RAW.tar 1.3 Gb (http)(custom) TAR (of BED, BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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