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Series GSE66886 Query DataSets for GSE66886
Status Public on Jun 12, 2017
Title The INO80 chromatin remodeling complex regulates the gene expression (no stress)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary RNA micro array analyses were performed using Arp5-, Arp8-knockout and Arp8-over expression Nalm-6 cells. As a result, various gene were up- or down-regulated in both Arp5- and Arp8-knockout cells. Because of these Arps are essential and specific component of the INO80 complex, it was quite possible that the INO80 complex was related to these gene expression.
 
Overall design The gene locus of Arp5 and Arp8 were disrupted by homologous recombination. In the case of Arp8-knockout cells, Arp8 over expression was caused by tetracycline-regulated (Tet-Off) system incorporation. Total RNAs were extracted and subjected to microarray analysis.
 
Contributor(s) Takahashi Y, Murakami H, Harata M
Citation(s) 28270832
Submission date Mar 13, 2015
Last update date Feb 22, 2018
Contact name Masahiko Harata
E-mail(s) labharata@gmail.com
Organization name Tohoku University
Department Graduate School of Agricultural Science
Lab Molecular Biology
Street address 468-1 Aramaki Aza Aoba, Aoba-ku
City Sendai
ZIP/Postal code 980-8572
Country Japan
 
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (4)
GSM1633762 WT
GSM1633763 Arp5-knockout
GSM1633764 Arp8-over expression
This SubSeries is part of SuperSeries:
GSE66888 The INO80 chromatin remodeling complex regulates the gene expression
Relations
BioProject PRJNA278168

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66886_RAW.tar 15.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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