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| Status |
Public on Nov 04, 2015 |
| Title |
ESRP2 Regulates A Conserved And Cell-Type-Specific Splicing Program to Support Postnatal Liver Maturation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Alternative splicing greatly expands the proteomic diversity but its functional impact is often unclear. Here, we identify a highly conserved and temporally coordinated cell-type-specific splicing program, which is activated in part by ESRP2 during postnatal liver development. Consistent with failure of many neonatal-to-adult splicing transitions, Esrp2 null mice exhibit persistent expression of fetal markers and loss of mature hepatocyte characteristics. Conversely, ectopic expression of ESRP2 in immature mouse or human hepatocytes results in a reciprocal switch in splicing. Our findings define an essential role for ESRP2 in generation of conserved repertoires of adult splice isoforms that facilitate postnatal liver maturation.
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| Overall design |
Mouse liver RNA was isolated with Trizol (Invitrogen). Hi-Seq libraries were prepared and paired-end 100bp Illumina sequencing was performed on mouse liver samples from different developmental stages.
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| Contributor(s) |
Kalsotra A, Xiao X |
| Citation(s) |
26531099 |
| |
| Submission date |
Mar 17, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jaegyoon Ahn |
| Organization name |
UCLA
|
| Department |
IBP
|
| Lab |
Xiao lab
|
| Street address |
611 Charles E. Young Drive
|
| City |
LA |
| State/province |
CA |
| ZIP/Postal code |
90095-1570 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA278684 |
| SRA |
SRP056267 |
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