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Series GSE67048 Query DataSets for GSE67048
Status Public on Sep 01, 2015
Title Identification of diffrentially expressed genes upon DDSR1 knockdown
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Upon DNA damage, the DNA damage response (DDR) elicits a complex signaling cascade, which includes the induction of multiple non-coding RNA species. Recently long non-coding RNAs (lncRNAs) have been shown to contribute to DDR by regulating gene expression. However, very little is known about the role that lncRNAs play in regulating DNA Repair. Using a genome-wide microarray screen we identified a novel ubiquitously expressed lncRNA, DDSR1 (DNA damage-sensitive RNA 1), which is induced upon DNA damage by several DNA double-strand break (DSB) agents. DDSR1 induction upon DNA damage is dependent on the ATM-NF-kB pathway but p53 independent. However, DDSR1 acts in the p53 network by negatively regulating p53 mediated gene expression. Loss of DDSR1 impairs cell proliferation, DDR signaling and reduces DNA repair capacity by homologous recombination (HR). The HR defect upon DDSR1 knockdown is due to reduced end resection caused by aberrant BRCA1 and RAP80 accumulation at DSB sites. In line with dual role of DDSR1 in gene regulation and HR, DDSR1 interacts with hnRNPUL1, an RNA-binding protein involved in transcription and HR. Our results reveal a previously unknown lncRNA involved in regulation of DDR by contributing to gene regulation and DNA repair by HR. Our findings highlight the importance of DDSR1 in maintaining genome stability and suggest that the DDR is even more complex than currently assumed.
We used microarrays to identify gene expression changes upon DDSR1 knockdown
 
Overall design hTERT immortalised Human Fibroblast cells were transfected a non specific siRNA or siRNA against DDSR1,72 hours post transfection RNA was isolated and process for Microarray. 3 Biological Replicates
 
Contributor(s) Sharma V, Misteli T
Citation(s) 26412854, 26697398
Submission date Mar 18, 2015
Last update date Dec 24, 2015
Contact name Vivek Sharma
E-mail(s) vivek.sharma@nih.gov
Phone 3014352672
Organization name NCI
Department CCR
Lab LRBGE
Street address 41 Library Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL19251 [HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [probe set (exon) version]
Samples (6)
GSM1637471 siNS1,Biological Replicate1
GSM1637472 siNS2,Biological Replicate2
GSM1637473 siNS3,Biological Replicate2
This SubSeries is part of SuperSeries:
GSE72358 A BRCA1 interacting lncRNA regulates homologous recombination
Relations
BioProject PRJNA278848

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67048_RAW.tar 71.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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