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| Status |
Public on May 17, 2016 |
| Title |
Deep sequencing identifies PD0325901 modulated microRNAs in the J1 mESCs |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
PD0325901 is involved in improving reprogramming efficiency during inducing pluripotent stem cells (iPSC) or maintain a blastocyst-like state in embryonic stem cells (ESC). However, the knowledge about this small molecule regulating miRNAs in ESC was limited. To understand the role of miRNAs during PD03-induced ESC maintenance and gain an insight how PD0325901 regulates miRNAs expression; we performed small RNA sequencing using Illumina HiSeq 2000 under the compounds treatment. The data show the miRNAs regulated by PD0325901.
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| Overall design |
J1 mESCs maintained in medium containing 1000 U/mL LIF and supplemented with PD0325901 for 24 hours, J1 treated with DMSO was set as control. Then total RNA was extracted for analysis.
|
| Web link |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4685126/
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| |
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| Contributor(s) |
Ai Z, Wu Y, Guo Z |
| Citation(s) |
26770202 |
| |
| Submission date |
Apr 03, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Zhiying Ai |
| E-mail(s) |
zhiying.ai@hotmail.com
|
| Organization name |
Northwest A&F University (NWAFU)
|
| Street address |
No.3 Taicheng Road, Yangling
|
| City |
xi'an |
| State/province |
shannxi |
| ZIP/Postal code |
712100 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA280279 |
| SRA |
SRP056857 |
