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Series GSE67807 Query DataSets for GSE67807
Status Public on Dec 05, 2017
Title Supplementation with estradiol cypionate at the onset of a synchronized proestrus alters the uterine gene expression of suckled anestrous beef cows
Organism Bos indicus
Experiment type Expression profiling by high throughput sequencing
Summary Purpouse: The aim was evaluated the impact of estradiol cypionate (ECP) supplementation at the onset of a proestrus [i.e. at progesterone (P4) device removal of the synchronization of ovulation protocol] on the global endometrial gene expression of suckled anestrous beef cows
Methods: A total of 12 suckled cows presenting absence of corpus luteum detected by ultrasound received 2 mg of estradiol benzoate im and an intravaginal P4 device. Eight days later, P4 devices were removed, and the cows received 500 mg of sodium cloprostenol IM. Cows were blocked according to body condition score and the diameter of largest follicle (LF) at P4 device removal and were randomly assigned to receive an im treatment with 1mg of ECP (ECP, n=6) or not (CON, n=6) at the P4 device removal. All cows received 10μg of buserelin acetate 48 h after the P4 device removal and were timed artificially inseminated immediately after. Cows presenting a new corpus luteum formed 6 d after the GnRH treatment had an endometrial fragment collected by transcervical biopsy
Results: The integrated analysis was performed using DAVID database. A total of 135 transcripts were differentially expressed between ECP and CON groups, of which 73 genes were up regulated by ECP suplmentation and 62 genes in the CON cows. Two pathways were overrepresented by the ECP-induced transcripts: pathways in cancer (n=5 genes) and small cell lung cancer (n=3 genes). On the other hand, ECP-inhibited transcripts indicated the enrichment of three pathways: Parkinson’s disease (n=3 genes), oxidative phosphorylation (n=3 genes) and Alzheimer’s disease (n=3 genes). More specifically, ECP-induced transcripts associated with pathways in cancer [gene symbol (fold change); respectively] were LAMC3 (1.55), PTCH1 (1.51), PTCH2 (1.52), PIK3R3 (1.22) and PIAS1 (1.18), whereas ECP-inhibited transcripts associated with oxidative phosphorylation were ATP5F1 (1.18), ATP5J (1.24) and NDUFB3 (1.37).
Conclusion: The ECP supplementation at onset of the synchronized proestrous slightly alters the uterine transcriptome.
Overall design endometrial mRNA profiles of endocrine manipulated cows were generated by deep sequencing using Illumina HiScanSQ platform
Contributor(s) Francisco de Sá Filho M, Mesquita F, Andrade S, Gasparin G, Gonella A, Lehmann Coutinho L, Binelli M
Citation(s) 29118976
Submission date Apr 13, 2015
Last update date May 15, 2019
Contact name Mario Binelli
Phone 19998591388
Organization name University of Sao Paulo (USP)
Department department of animal reproduction
Lab Molecular Physiology and Endocrinology
Street address Rua Duque de Caxias Norte, 255
City Pirassununga
State/province SP
ZIP/Postal code 13635-900
Country Brazil
Platforms (1)
GPL19671 Illumina HiScanSQ (Bos indicus)
Samples (12)
GSM1656394 1814
GSM1656395 B1042
GSM1656396 E0040
BioProject PRJNA280965
SRA SRP057108

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Supplementary file Size Download File type/resource
GSE67807_Counts_Manoel.xlsx 1.7 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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