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Series GSE67834 Query DataSets for GSE67834
Status Public on Mar 15, 2017
Title Transcriptome analysis of thymic APC subsets, mTECs and thymic DCs in comparison to splenic DCs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Thymic antigen-presenting cells (APCs), including thymic dendritic cells (t-DCs) and medullary thymic epithelial cells (mTECs) have been described to play a critical role in thymic Treg generation. Our findings could show that both these thymic APCs can induce a more pronounced demethylation of Foxp3 and other Treg-specific epigenetic signature genes in developing Tregs when compared to splenic DCs. In order to elucidate the unique properties of thymic APCs, gene expression profiling was performed in comparison to splenic DCs. Transcriptome analysis of thymic APCs revealed differential expression of costimulatory molecules that could be involved in stable Treg generation. Importantly, both mTEC- and t-DC- induced alloantigen-specific Tregs displayed significantly higher efficacy in prolonging skin allograft acceptance when compared to alloantigen-specific Tregs generated by splenic DCs.
Overall design Thymic APCs, including mTECs and t-DCs and splenic DCs were isolated ex vivo from thymus as CD45-EpCAM+Ly51- (mTECs) and CD45+EpCAM-CD11chiLin- (t-DCs) and from spleen as CD11chiLin- (splenic DCs) (Lin is defined as CD90, CD49b, F4/80 and CD19), respectively.
Contributor(s) Garg G, Huehn J
Citation(s) 28415767
Submission date Apr 14, 2015
Last update date May 15, 2019
Contact name Michael Beckstette
Organization name Helmholtz Centre for Infection Research
Department Molecular Infection Biology
Lab Experimental Immunology
Street address Inhoffenstrase 7
City Braunschweig
State/province Lower Saxony
ZIP/Postal code 38124
Country Germany
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (9)
GSM1656990 mTECs_1
GSM1656991 mTECs_2
GSM1656992 mTECs_3
BioProject PRJNA281042
SRA SRP057134

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67834_DESeq2_genes_diffexp_by_fc_mTECs_vs_sp-DCs.csv.gz 4.9 Mb (ftp)(http) CSV
GSE67834_DESeq2_genes_diffexp_by_fc_mTECs_vs_t-DCs.csv.gz 4.9 Mb (ftp)(http) CSV
GSE67834_DESeq2_genes_diffexp_by_fc_sp-DCs_vs_mTECs.csv.gz 4.9 Mb (ftp)(http) CSV
GSE67834_DESeq2_genes_diffexp_by_fc_sp-DCs_vs_t-DCs.csv.gz 4.3 Mb (ftp)(http) CSV
GSE67834_DESeq2_genes_diffexp_by_fc_t-DCs_vs_mTECs.csv.gz 4.9 Mb (ftp)(http) CSV
GSE67834_DESeq2_genes_diffexp_by_fc_t-DCs_vs_sp-DCs.csv.gz 4.3 Mb (ftp)(http) CSV
GSE67834_RAW.tar 4.0 Mb (http)(custom) TAR (of RPKM, TAB)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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