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Series GSE68068 Query DataSets for GSE68068
Status Public on Apr 21, 2015
Title Investigating the effect of CodY mutation on the gene expression in Streptococcus suis serotype 2 SC19 strain.
Organism Streptococcus suis
Experiment type Expression profiling by array
Summary To investigate the effect of CodY mutation on the gene expression in Streptococcus suis serotype 2 SC19 strain, we have employed whole genome microarray expression profiling as a discovery platform to identify genes regulated by CodY mutation. DNA microarray analysis was performed using an Agilent custom-designed oligonucleotide microarray. Based upon the whole genome sequence of SC19 , specific 60-mer oligonucleotide probes were designed using eArray (https://earray.chem.agilent.com/earray/), to cover all annotated genes. Probes were printed seven times on microarray slides. Three biological replicates of total RNA from two wild type strains and from two codY mutant strains were amplified and labeled with Cy3-CTP using Low Input Quick Amp Labeling Kit, one-color(Agilent technologies, US), following the manufacturer’s instructions. Labeled cRNA was purified using the RNeasy mini kit (Qiagen). After fragmentation, microarray slides were hybridized with 600 ng Cy3-labeled cRNA. Hybridization was performed at 65 °C for 17 h with rotation at 10 rpm. Microarray slides were washed and scanned by an Agilent Microarray Scanner (G2565BA). Those genes with greater than two-fold change ratios were regarded as differentially expressed genes.
 
Overall design codY mutation induced gene expression in Streptococcus suis serotype 2 SC19 was detected in two wild type and two codY mutated strain of Streptococcus suis serotype 2.
 
Contributor(s) Bei W, Feng L
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Submission date Apr 20, 2015
Last update date Apr 22, 2015
Contact name liping feng
E-mail(s) flp2013@foxmail.com
Organization name Shanghai Laboratory Animal Research Center
Street address No. 3577 Jinke Rd., Shanghai
City shanghai
ZIP/Postal code 201203
Country China
 
Platforms (1)
GPL20080 Agilent-022267 Streptococcus suis
Samples (2)
GSM1662248 △codY_1
GSM1662249 WT_1
Relations
BioProject PRJNA281651

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68068_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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