 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 17, 2007 |
| Title |
Expression data for reproductive development in rice |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
|
| Summary |
Plants initially undergo a period of vegetative development, in which it produces leaves from shoot apical meristem (SAM) and roots from the root apical meristem. Later in development, the SAM undergoes a change in fate and enters reproductive development called as floral transition, producing flowers and seeds. Our understanding of the molecular and genetic mechanisms that underlie reproductive development in plants has increased tremendously in the past decade, essentially through the work on Arabidopsis. In this study, we have analyzed the spatial and temporal gene expression in various tissues/organs and developmental stages of rice using microarray technology to identify the genes differentially expressed during various stages of reproductive development.
Keywords: Development time course
|
| |
|
| Overall design |
Various rice tissues/organs (seedling root, mature leaf and Y leaf) and stages of reproductive (panicle and seed) development were used for RNA extraction and hybridization on Affymetrix microarrays. Three biological replicates of each sample were used for microarray analysis. We sought to obtain developmental and temporal gene expression profiles at each stage to identify the differentially expressed genes. Different stages of panicle and seed development have been categorized according to panicle length and days after pollination, respectively, based on landmark developmental events as follows: up to 0.5 mm, shoot apical meristem and rachis meristem (SAM); 0-3 cm, floral transition and floral organ development (P1); 3-10 cm, meiotic stage (P2 and P3); 10-15 cm, young microspore stage (P4); 15-22 cm, vacuolated pollen stage (P5); 22-30 cm, mature pollen stage (P6); 0-2 dap, early globular embryo (S1); 3-4 dap, middle and late globular embryo (S2); 5-10 dap, embryo morphogenesis (S3); 11-20 dap, embryo maturation (S4); 21-29, dormancy and desiccation tolerance (S5). These stage specifications are approximations based on information from Itoh et al., 2005, Plant Cell Physiol, 46, 23-47.
|
| |
|
| Contributor(s) |
Kapoor S, Tyagi AK, Khurana JP, Arora R, Agarwal P, Nijhawan A, Sharma P, Jain M |
| Citation(s) |
17293439, 19490115, 19788421, 21554676, 24398598, 23638098, 22363522, 20637108, 26134707, 17640358 |
| |
| Submission date |
Jan 29, 2007 |
| Last update date |
Apr 16, 2020 |
| Contact name |
Sanjay Kapoor |
| E-mail(s) |
kapoors@genomeindia.org
|
| Phone |
91 11 65171135
|
| URL |
http://www.dpmb.ac.in/
|
| Organization name |
University of Delhi South Campus
|
| Department |
Department of Plant Molecular Biology
|
| Street address |
Benito Juarez Road
|
| City |
New Delhi |
| State/province |
Delhi |
| ZIP/Postal code |
110021 |
| Country |
India |
| |
|
| Platforms (1) |
| GPL2025 |
[Rice] Affymetrix Rice Genome Array |
|
| Samples (45)
|
| GSM159177 |
Root, 7-day-old Seedling, biological rep 1 |
| GSM159178 |
Root, 7-day-old Seedling, biological rep 2 |
| GSM159179 |
Root, 7-day-old Seedling, biological rep 3 |
| GSM159180 |
Mature leaf, biological rep 1 |
| GSM159181 |
Mature leaf, biological rep 2 |
| GSM159182 |
Mature leaf, biological rep 3 |
| GSM159183 |
Y Leaf, biological rep 1 |
| GSM159184 |
Y Leaf, biological rep 2 |
| GSM159185 |
Y Leaf, biological rep 3 |
| GSM159186 |
SAM, biological rep 1 |
| GSM159187 |
SAM, biological rep 2 |
| GSM159188 |
SAM, biological rep 3 |
| GSM159189 |
Young inflorescence (P1, upto 3 cm), biological rep 1 |
| GSM159190 |
Young inflorescence (P1, upto 3 cm), biological rep 2 |
| GSM159191 |
Young inflorescence (P1, upto 3 cm), biological rep 3 |
| GSM159192 |
Inflorescence (P2, 3 - 5 cm), biological rep 1 |
| GSM159193 |
Inflorescence (P2, 3 - 5 cm), biological rep 2 |
| GSM159194 |
Inflorescence (P2, 3 - 5 cm), biological rep 3 |
| GSM159195 |
Inflorescence (P3, 5 - 10 cm), biological rep 1 |
| GSM159196 |
Inflorescence (P3, 5 - 10 cm), biological rep 2 |
| GSM159197 |
Inflorescence (P3, 5 - 10 cm), biological rep 3 |
| GSM159198 |
Inflorescence (P4, 10 - 15 cm), biological rep 1 |
| GSM159199 |
Inflorescence (P4, 10 - 15 cm), biological rep 2 |
| GSM159200 |
Inflorescence (P4, 10 - 15 cm), biological rep 3 |
| GSM159201 |
Inflorescence (P5, 15 - 22 cm), biological rep 1 |
| GSM159202 |
Inflorescence (P5, 15 - 22 cm), biological rep 2 |
| GSM159203 |
Inflorescence (P5, 15 - 22 cm), biological rep 3 |
| GSM159204 |
Inflorescence (P6, 22 - 30 cm), biological rep 1 |
| GSM159205 |
Inflorescence (P6, 22 - 30 cm), biological rep 2 |
| GSM159206 |
Inflorescence (P6, 22 - 30 cm), biological rep 3 |
| GSM159207 |
Seed (S1, 0 - 2 dap), biological rep 1 |
| GSM159208 |
Seed (S1, 0 - 2 dap), biological rep 2 |
| GSM159209 |
Seed (S1, 0 - 2 dap), biological rep 3 |
| GSM159210 |
Seed (S2, 3 - 4 dap), biological rep 1 |
| GSM159211 |
Seed (S2, 3 - 4 dap), biological rep 2 |
| GSM159212 |
Seed (S2, 3 - 4 dap), biological rep 3 |
| GSM159213 |
Seed (S3, 5 - 10 dap), biological rep 1 |
| GSM159214 |
Seed (S3, 5 - 10 dap), biological rep 2 |
| GSM159215 |
Seed (S3, 5 - 10 dap), biological rep 3 |
| GSM159216 |
Seed (S4, 11 - 20 dap), biological rep 1 |
| GSM159217 |
Seed (S4, 11 - 20 dap), biological rep 2 |
| GSM159218 |
Seed (S4, 11 - 20 dap), biological rep 3 |
| GSM159219 |
Seed (S5, 21 - 29 dap), biological rep 1 |
| GSM159220 |
Seed (S5, 21 - 29 dap), biological rep 2 |
| GSM159221 |
Seed (S5, 21 - 29 dap), biological rep 3 |
|
| Relations |
| BioProject |
PRJNA98537 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6893_RAW.tar |
199.4 Mb |
(http)(custom) |
TAR (of CEL) |
|
|
|
|
 |
Less...
More...