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Series GSE69547 Query DataSets for GSE69547
Status Public on Jun 03, 2015
Title Otx2 controls regional patterning within the MGE and regional identity of the septum [Agilent]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The Otx2 homeobox transcription factor is essential for gastrulation and early neural development. We generated Otx2 conditional knockout (cKO) mice to investigate its roles in telencephalon development after E9.0. We conducted transcriptional profiling and in situ hybridization to identify genes de-regulated in Otx2 cKO ventral forebrain. In parallel, we used ChIP-seq to identify enhancer elements, OTX2 binding motif, and which de-regulated genes are likely direct targets of Otx2 transcriptional regulation. We found that Otx2 was essential in septum specification; regulation of Fgf signaling in the rostral telencephalon; and medial ganglionic eminence (MGE) patterning, neurogenesis, and oligodendrogenesis. Within the MGE, Otx2 was required for ventral but not dorsal identity; this is the first demonstration of a transcription factor that contributes to regional patterning within the MGE.
Overall design We used an Otx2flox (Otx2f) allele with RxCre, Cre-line, to abrogate Otx2 expression ~E8.5 and ~E10.0. RxCre mice express Cre throughout the forebrain, except in the caudal dorsomedial domain that includes the choroid plexus and cortical hem. We compared mRNA expression in the ganglionic eminences and septum of RxCre conditional knock out (cKO) and control mouse embryos at E12.5.
Contributor(s) Hoch RV, Lindtner S, Price JD, Rubenstein JR
Citation(s) 26166575
Submission date Jun 03, 2015
Last update date Jan 12, 2017
Contact name John Rubenstein
Organization name UCSF
Department Psychiatry
Lab Rubenstein Lab
Street address 1550 4th Street
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (7)
GSM1703681 Basal Ganglia_E12.5_Control_Rep1
GSM1703682 Basal Ganglia_E12.5_Control_Rep2
GSM1703683 Basal Ganglia_E12.5_Control_Rep3
This SubSeries is part of SuperSeries:
GSE69727 Otx2 controls regional patterning within the MGE and regional identity of the septum
BioProject PRJNA285819

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69547_RAW.tar 15.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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