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Series GSE7 Query DataSets for GSE7
Status Public on Jul 30, 2001
Title Tryptophan metabolism response in E. coli
Organism Escherichia coli
Experiment type Expression profiling by array
Summary DNA microarray analysis of gene expression in response to physiological and genetic changes that affect tryptophan metabolism in Escherichia coli.
We investigated the global changes in mRNA abundance in Escherichia coli elicited by various perturbations of tryptophan metabolism. To do so we printed DNA microarrays containing 95% of all annotated E. coli ORFs. We determined the expression profile that is predominantly dictated by the activity of the tryptophan repressor. Only three operons, trp, mtr, and aroH, exhibited appreciable expression changes consistent with this profile. The quantitative changes we observed in mRNA levels for the five genes of the trp operon were consistent within a factor of 2, with expectations based on established Trp protein levels. Several operons known to be regulated by the TyrR protein, aroF-tyrA, aroL, aroP, and aroG, were down-regulated on addition of tryptophan. TyrR can be activated by any one of the three aromatic amino acids. Only one operon, tnaAB, was significantly activated by the presence of tryptophan in the medium. We uncovered a plethora of likely indirect effects of changes in tryptophan metabolism on intracellular mRNA pools, most prominent of which was the sensitivity of arginine biosynthetic operons to tryptophan starvation.
This study is detailed in Khodursky AB et al.(2000) Proc Natl Acad Sci U S A 97:12170-5
Keywords: other
Contributor(s) Khodursky AB, Peter BJ, Cozzarelli NR, Botstein D, Brown PO, Yanofsky C
Citation(s) 11027315
Submission date Jul 30, 2001
Last update date Feb 23, 2012
Organization Stanford Microarray Database (SMD)
Phone 650-498-6012
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (2)
GPL16 ecoli_3.0
GPL17 ecoli_4.3
Samples (29)
GSM513 tnaA2,W3110 minimal +Trp 50ug/ml vs. dnaC genomic DNA
GSM514 trpR2tnaA2,W3110 minimal +Trp 50ug/ml vs. dnaC genomic DNA
GSM515 5' vs 0' in minimal medium +0.2% glu
BioProject PRJNA85445

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